Karla,
We try to run 1/2 of the required cases with known negative cases and the other
1/2 with known positive cases. In addition, all cases are run with a negative
reagent control in place of the antibody using the optimized protocol for the
antibody. These are your negative reagent
I have used the CAP published 'Quality Management in Anatomic Pathology' and it
has been very helpful with this:
For a well-characterized antibody with a limited spectrum of antigenic
targets, like chromogranin, or prostate specific antigen, the validation can be
limited. A panel of 10
I had to revalidate all of my antibodies when we changed our IHC
platform. I used store bought TMAs and passed my CAP inspection with
flying colors. The cost savings is enormous. Ideally, they should be
processed the same as your test tissue, but since our CAP proficiency
slides are not, I did
Braud tbr...@holyredeemer.com
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الموضوع: [Histonet] RE: IHC Validation with TMAs
I had to revalidate all of my antibodies when we changed our IHC
platform. I used store bought TMAs and passed my CAP inspection
: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud
Sent: Monday, August 04, 2014 1:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: IHC Validation with TMAs
I had to revalidate all of my antibodies when we changed
Thank you to everyone who sent a template. This is very helpful. I do
need clarfication on the 10 cases that are required for validation. Do you
include the 10 cases on this same form, or do you have something separate
for that? These look ideal for optimization. From what I have read
online,
I document all of the worksheet process¹ and then I create a ³Validation
Report² where my technical director both reviews all slides and signs this
report.
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com
On
Are you changing the antibody dilution? If so, I would run 2-3 positive and
negative cases and make sure that the immunoreactivity compares favorably to
what you observed before.
Richard
Richard W. Cartun, MS, PhD
Director, Histology Immunopathology
Director, Biospecimen Collection Programs
On my IHC validation documents I have a space for comments. If I change
anything in a protocol I make a note in the comments space. I haven't had any
issue with any accrediting agencies.
Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu
Sent: Friday, March 15, 2013 1:05 PM
Subject: [Histonet] RE: IHC validation
Laurie, for first time validation use controls. Don't use patient cases that
are done for initial diagnostics. If they are repeats to test the system, that
is ok.
The question
Laurie, for first time validation use controls. Don't use patient cases that
are done for initial diagnostics. If they are repeats to test the system, that
is ok.
The question is, who tested the controls? Ideally you will send a
representative sample of your slides to another lab that uses the
All you need to do is compare the new clone to your current clone and show the
new clone is concordant with the new clone for sensitivity and specificity .
You don't need to compare to the same clone elsewhere.
Tim Morken
Department of Pathology
UC San Francisco Medical Center
505 Parnassus
Thank you all for the info. I agree that in-house validation is the way
to go. The only reason we had been using an outside reference lab was
due to recommendations during a JC inspection which were applied to our
area, but not really meant for IHC. The inspector was not a histology
person, so
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dessoye,
Michael J
Sent: Friday, May 04, 2012 9:05 AM
To: Morken, Timothy; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: IHC Validation
Thank you
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