Why would you like to avoid detection of intracellular Abeta inclusions?
//Daniel
On Wed, Oct 26, 2011 at 8:51 AM, Hobbs, Carl wrote:
>
> Be careful: HIER may expose not only extracellular amyloid plaques but also
> intracellular APP ( or...is it intracellular A beta ;-))
> Eg: with 6E10.
Be careful: HIER may expose not only extracellular amyloid plaques but also
intracellular APP ( or...is it intracellular A beta ;-))
Eg: with 6E10 it is directed against an epitope found in APP and also A
beta: if you do Formic acid, only extracellular plaques are positive...after
HIE
We have success with 6E10 and 4G8 clones using citrate retrieval.
Brett
Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803
-Original Message-
From: histonet-boun...@list
We use Dako's Beta Amyloid with high pH heat retrieval. Works very well.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histonet-boun...@lists.utsouthwestern.edu
