From: Bob Richmond rsrichm...@gmail.com
To: Tony Reilly tony_rei...@health.qld.gov.au
Cc: histonet@lists.utsouthwestern.edu
Sent: Wednesday, February 27, 2013 8:12 PM
Subject: Re: [Histonet] Re: Unencased Amoeba Stain
You're referring I suppose to the Gomori-Wheatley modification
Matt Brooks asks: One of our Neuropath docs is inquiring about a
special stain for unencased
[amoebae] in cornea biopsies. I did a search and Gridley's method was
the best option that appreaded [sic]. Is there someone that could
either verify this stain will work on this organism or let me know
Hi Matt
The Heidenhain's stain is good but if you want one that is prettier try
Gomori's one step trichrome. I have only ever used it for intestinal
protozoa but I think it would work for all.
regards
Tony
Tony Reilly B.App.Sc. , M.Sc.
Chief Scientist, Anatomical Pathology
Pathology
You're referring I suppose to the Gomori-Wheatley modification of
the one step trichrome, used for staining intestinal amoebae. Don't
know if it will work on tissue sections. It has quite a number of
variants.
Once again, I'd advise consulting an eye pathologist.
Bob Richmond
Samurai Pathologist
It is true that I have never used it on tissue sections as I have
usually performed it on smears for the Microbiology department.
However some of the specimens stained were formalin fixed and stained
well while others were PVA fixed.
regards
Tony
Tony Reilly B.App.Sc. , M.Sc.
Chief
It's better not to assume that a stain developed for smears will work
on tissue sections without modification. The Gram stain is an obvious
case in point.
Bob Richmond
Samurai Pathologist
Maryville TN
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On Wed, Feb 27, 2013 at 9:20 PM, Tony Reilly
