[Histonet] RE: Xylene Substitute Protocols

2014-07-25 Thread Vanessa Avalos
Statlab XS-3 & Acrymount. I do H & E staining only. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hilliard, Dawud Deshawn Sent: Thursday, July 24, 2014 9:50 AM To: histonet@lists.utsouthwestern.edu Subj

[Histonet] RE: Xylene Substitute Protocols

2014-07-24 Thread Shannon Kleiner
Richard Allen mounting media REF 4111. >>> Shannon Kleiner 7/24/2014 12:59 PM >>> Formula 83 in first three stations and last four stations on the Leica XL stainer. >>> "Wineman, Terra" 7/24/2014 12:06 PM >>> We use Richard Allan Clear-Rite 5min * 3 stations with any xylene based mountin

[Histonet] RE: Xylene Substitute Protocols

2014-07-24 Thread Shannon Kleiner
Formula 83 in first three stations and last four stations on the Leica XL stainer. >>> "Wineman, Terra" 7/24/2014 12:06 PM >>> We use Richard Allan Clear-Rite 5min * 3 stations with any xylene based mounting type Terra Wineman, HTL(ASCP)cm Novus International -Original Message- Fro

[Histonet] RE: Xylene Substitute Protocols

2014-07-24 Thread Wineman, Terra
We use Richard Allan Clear-Rite 5min * 3 stations with any xylene based mounting type Terra Wineman, HTL(ASCP)cm Novus International -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hilliard, Dawud Deshaw

[Histonet] RE: Xylene substitute for processing GI biopsies

2014-04-21 Thread Donna Millard
Joe, Yes. We are about to go xylene-free (though we are still planning on using if for cleaning, because the feedback we got was that we still needed to clean the processors with xylene or we would run into problems, and my supervisor heard from people that they had to go back to xylene flushing

Re: [Histonet] RE: xylene substitute for processing

2012-03-07 Thread Jennifer MacDonald
2 07:58 AM To "'Masood, Sameena Kathryn'" , "Cross, Kelly" , "Histonet Listserv (E-mail) (histonet@lists.utsouthwestern.edu)" cc Subject [Histonet] RE: xylene substitute for processing Some people have issues with the smell and the other option is Ana

Re: [Histonet] RE: xylene substitute for processing

2012-03-07 Thread Grantham, Andrea L - (algranth)
I have been using Clear Rite 3 for years on animal tissue in a VIP for overnite processing and it is great. I get it from Fisher. There has never been a problem with special stains but I use xylene on my stain lines. Andi Grantham On Mar 7, 2012, at 8:35 AM, Masood, Sameena Kathryn wrote: >

[Histonet] RE: xylene substitute for processing

2012-03-07 Thread Marcum, Pamela A
estern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Masood, Sameena Kathryn Sent: Wednesday, March 07, 2012 9:36 AM To: Cross, Kelly; Histonet Listserv (E-mail) (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: xylene substitute for processing Kelly, Histo-Clear can b

[Histonet] RE: xylene substitute for processing

2012-03-07 Thread Masood, Sameena Kathryn
Kelly, Histo-Clear can be directly substituted for Xylene at every point in processing and staining. It's completely non-toxic (even has a food grade rating), is made from oranges, and smells delicious. You don't have to work in a hood and it seems to make tissue less brittle than Xylene also.

[Histonet] RE: xylene substitute for processing

2012-03-05 Thread Vanessa Avalos
I use the Shandon Excelsior and use an overnight run with Iso Alcohol and only use Xylene in the cleaning cycle. Our Thermo Rep came out and set it all up for us. No problems with the processing. I also use sub xylene in staining with a few extra steps but no problems. -Original Message---

[Histonet] re: xylene substitute for processing

2012-03-05 Thread Martin, Erin
We have been processing without xylene on ThermoFisher Excelsior processors for a couple of years. We go directly from 100% isopropyl alcohol into paraffin. A thermo sales rep should be able to give you some literature on this. When we first got the processors we did side by side validation

Re: [Histonet] Re: xylene substitute

2010-10-27 Thread Jennifer MacDonald
xylene substitutes also require more time than xylene "Johnson, Teri" Sent by: histonet-boun...@lists.utsouthwestern.edu 10/27/2010 10:13 AM To "'histonet@lists.utsouthwestern.edu'" cc Subject [Histonet] Re: xylene substitute Hi all, I just wanted

[Histonet] RE: xylene substitute

2010-10-26 Thread Nathan Jentsch
We use a product called pro-par, produced by Anatech. We don't use permount routinely, but a few of our specials require it. We dehydrate and then clear with the pro-par, and it works fine for those stains. It is much less harsh on tissue than xylene; we have three VIP's, and our specimens sit i

[Histonet] Re: Xylene Substitute

2009-10-24 Thread Robert Richmond
Sheila Hass at Micro Path Laboratories [where?} asks: >>Could someone recommend a xylene substitute and mounting media to use for staining and coverslipping (no vendors please)? I am considering processing without xylene, and would like to discontinue it everywhere if possible.<< We've discussed t

[Histonet] Re: Xylene substitute

2009-04-13 Thread Robert Richmond
You can view the MSDS for CBG Biotech's "Formula 83" xylene substitute at www.cbgbiotech.com/msds/MSDS-Formula83071007.pdf It's described as a "naphthenic hydrocarbon blend". I'd note the low flash point (7 C, 45 F), considerably lower than xylene's. Other aliphatic or naphthenic (cycloalkane) m

[Histonet] RE: Xylene substitute for automated coverslipper

2009-02-12 Thread Blazek, Linda
Jim, I use Formula 83 from CBG on a Richard Allan CTM 6 glass coverslipper with no problems. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 293-4424 ext 7118 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.u

Re: [Histonet] Re: Xylene substitute

2009-01-23 Thread Victor Tobias
estern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, January 22, 2009 9:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Xylene substitute Questions about xylene substitutes: This has been discussed a good many times on Histonet.

RE: [Histonet] Re: Xylene substitute

2009-01-23 Thread Blazek, Linda
tonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, January 22, 2009 9:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Xylene substitute Questions about xylene substitutes: This has been discussed a good man

[Histonet] Re: Xylene substitute

2009-01-22 Thread Robert Richmond
Questions about xylene substitutes: This has been discussed a good many times on Histonet. There are two classes of xylene substitutes: Limonene and turpentine are terpenes. Several brands; AmeriClear was mentioned. Limonene is prepared by steam distillation of orange peels. It has a strong citru

[Histonet] RE: Xylene substitute

2009-01-22 Thread Blazek, Linda
Tom, We are using Formula 83 from CBG and like it a lot. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@l