Hi Julie,
Try increasing your deparaffinization times, sometimes there is just not enough
time in xylene so subsequent staining can be very inconsistent like described.
Kari Kienitz HT, (ASCP)
Histology Laboratory
Gastroenterology-EAST
The Oregon Clinic
NE 99th Ave
Portland, OR 97220
Julie,
Are the inconsistencies consistent among different sections? In other words,
with additional sections, are you seeing the paler areas in the same places or
different ones? If you section it today, are the pale areas in the same place
as when you sectioned it previously?
If it is the
