[Histonet] Re: processing mouse seminal vesicles

Try this processing schudule for mouse tissue:   70% 15 min 95% 15 min 95% 15 min 100% 15 min 100% 15 min 100% 15 min 100% 15 min Xylene 15 min Xylene 15 min Paraffin 15 min Paraffin 15 min Paraffin 15 min Paraffin 15 min   Good luck! Susan Bincsik         Does anybody have a protocol for this?  My

[Histonet] Re: Processing mouse seminal vesicles

Kathleen, Before changing your entire processing set-up, try decreasing the time in your alcohols and xylene to 10-15 minutes per station. Mouse seminal vesicles are very small and you don't need such rigorous dehydration and clearing to get proper processing. Teri Johnson, HT(ASCP)QIHC Managi