Re: [Histonet] Repeats in IHC

2018-10-29 Thread Terri Braud via Histonet
-- Message: 4 Date: Sat, 27 Oct 2018 13:48:06 + (UTC) From: Rene J Buesa To: "Kurth Virginia L." , "histonet@lists.utsouthwestern.edu" Subject: Re: [Histonet] REPEATS IN IHC Message-ID: <1773692175.85882.1540648086...@mail.yahoo.com> Content-Type:

Re: [Histonet] REPEATS IN IHC

2018-10-27 Thread Rene J Buesa via Histonet
Ginny:It seems you are asking for a "problem" average and that will vary per laboratory and how the whole process is carried out.Section washed out from the slide usually is consequence of poor processing or sectioning and has nothing to do with IHC, although, perhaps, a wrongly performed HIER

[Histonet] REPEATS IN IHC

2018-10-26 Thread Kurth Virginia L. via Histonet
Hello, I was wondering if anyone in the histology laboratory (IHC) has a limit on how many times one would repeat a stain; for example but not Limited too: The tissue fell off, negative control, wrong block,etc.. for IHC DAB and/or ISH. Thanks Ginny