[Histonet] Reticulin stain for poorly processed tissue

I am working on a project and have received slides containing tissue that seem to be poorly processed. I am currently using a Chandler's Reticulin stain kit that was purchased through American MasterTech. My liver control on the test slide is working but the tissue of unknown processing is not

Re: [Histonet] Reticulin Stain

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Re: [Histonet] Reticulin Stain

Hi All Here in our lab we routinely hand stain reticulin sections. Our preferred choice is the Gordon and Sweet method. The technique is easy to perform, diffing out can be easily controlled via the staining microscope and a variety of counterstains can be used in addition to the usual Neutral

Re: [Histonet] Reticulin Stain

Gomori's René On Thursday, July 21, 2016 12:04 PM, Anita Buchiane via Histonet wrote: Does anyone out there still do the retic by hand?  If so, can you share which procedure you use?  Thanks

[Histonet] Reticulin Stain

Does anyone out there still do the retic by hand? If so, can you share which procedure you use? Thanks ___ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for

[Histonet] Reticulin Stain(Gomori)

Dear Histonetters! I was wondering if anyone has a good working Gomori stain protocol for Reticulin fibers. i understand that it's a pretty routine procedure; however no one in my lab has ever done it, so I'm wondering if there is a ready-to-use kit already or is it all from scratch. I'm trying to

Re: [Histonet] Reticulin Stain(Gomori)

Polyscientific of Bayshore NY has a very simple kit. You can find them on line. Vikki On Jun 13, 2011 4:37 PM, Igor Deyneko igor.deyn...@gmail.com wrote: Dear Histonetters! I was wondering if anyone has a good working Gomori stain protocol for Reticulin fibers. i understand that it's a pretty

[Histonet] Reticulin Stain

Hello to all in histoland. We are having a problem with our reticulin stain. It is not showing a real delination of the reticulin fibers. They are there but it tends to fade off.We do the brown and hopps stain. Any help would be appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ

Re: [Histonet] Reticulin Stain

Hi Alison, Do you have a problem with all type of tissue ( Liver, Lung, Lymph Nodes, Trephine ... ) or just Trephine specimens. ? If you only have problems with Retic on BMT, if you use Formic Formaldehyde Decal fluid, see if EDTA make a difference. If you experience issue with all type of

Re: [Histonet] Reticulin Stain

We've had problems in the past when the reducing agent is not made fresh. Scott, Allison D allison_sc...@hchd.tmc.edu Sent by: histonet-boun...@lists.utsouthwestern.edu 03/23/2010 01:39 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Reticulin Stain Hello to all

Re: [Histonet] Reticulin stain Gomori

Message - From: Gudrun Lang gu.l...@gmx.at Date: Thursday, February 18, 2010 9:07 Subject: [Histonet] reticulin stain Gomori To: histonet@lists.utsouthwestern.edu Hi all! Today I was asked if I knew the chemistry behind the ammonia- iron-sulfat NH4Fe(SO4)2 . 12H2O reaction in the reticulin

[Histonet] reticulin stain Gomori

Hi all! Today I was asked if I knew the chemistry behind the ammonia-iron-sulfat NH4Fe(SO4)2 . 12H2O reaction in the reticulin stain of Gomori. I couldn't help so I forward the question to the histonet comunity. I hope, someone has a suggestion for me. Gudrun Lang

[Histonet] reticulin stain

Derek: I am sending it to you under separate cover. René J. --- On Fri, 6/19/09, Derek Papalegis derek.papale...@tufts.edu wrote: From: Derek Papalegis derek.papale...@tufts.edu Subject: [Histonet] reticulin stain To: histonet@lists.utsouthwestern.edu Date: Friday, June 19, 2009, 9:11 AM I am