but almost opposite
staining properties, which have been quite well understood since about 1880.
John Kiernan
Anatomy, UWO
London, Canada
= = =
- Original Message -
From: sk...@illinois.edu
Date: Monday, December 21, 2009 14:26
Subject: [Histonet] Sanderson's rapid bone stain + Acid
Hi All -
I am trying to figure out if the combination of Sanderson's rapid bone stain
and acid fuchsin as the counterstain (or methylene blue + basic fuchsin)
results in pink/red staining of biologically formed apatite (apatite not
associated with collagen produced by osteoblasts)? An
Regarding the counterstain for use with Sanderson's Rapid Bone Stain,
I use the Van Gieson picrofuchsin in that it will stain older, mature,
mineralized bone red. As an additional consequence of the VG
counterstain, the originally blue stained dense collagen (new bone/
osteoid) from the
Hi all,
A couple of weeks ago I posted a message about needing a new supplier
for Sanderson's RBS now that Surgipath/Leica have stopped selling it.
I've just had confirmation that Dorn Hart Microedge, Inc in Chicago
will be taking over the manufacture and supply of Sanderson's RBS. Stain
It was good hear that RBS was being picked up and sold by Dorn and Hart, but
you can also make this up yourself in the laboratory. Cathy
Sanderson/Mayton found a way to make this up easier than the original
formula and Surgipath manufactured it.
The original name was Stevenels Blue and the
Dear Fellow Histonetters,
There are 3 counterstains that can be used with the Sanderson's Rapid Bone
Stain (RBS). We primarily used the acidified acid fuchsin, however, we had
clients that only used a van Gieson counterstain. Light green can also be
used. We normally stained for 1 minute
-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cathy Mayton
Sent: Thursday, March 19, 2009 2:33 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Sanderson's Rapid Bone Stain
Dear Fellow Histonetters,
There are 3 counterstains that can
We are planning on trying this stain on our plastics. Has anyone tried it on
paraffin slides? If so, what were the results?
Thanks in advance for any help.
Suzanne
___
Suzanne Bruce, R.V.T.
Histologist / Necropsy Coordinator
@lists.utsouthwestern.edu
Subject: [Histonet] Sanderson's Rapid Bone Stain
We are planning on trying this stain on our plastics. Has anyone tried it on
paraffin slides? If so, what were the results?
Thanks in advance for any help.
Suzanne
___
Suzanne Bruce
Hello, I posted earlier regarding the Sanderson's Rapid Bone stain. I forgot
to mention that I will be using this stain on 75-100 micron thick ground
sections embedded in Technovit 7200 plastic.
I am also wondering about paraffin staining using this stain.
Here's one response so far:
What is the tissue? What are your endpoints?
Date: Wed, 18 Feb 2009 16:45:46 -0500
From: sbr...@vetpathservicesinc.com
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Sanderson's Rapid Bone stain
Hello, I posted earlier regarding the Sanderson's Rapid Bone stain. I forgot
.
From: Jack Ratliff [mailto:ratliffj...@hotmail.com]
Sent: Wed 2/18/2009 5:24 PM
To: Suzanne Bruce; Histonet
Subject: RE: [Histonet] Sanderson's Rapid Bone stain
What is the tissue? What are your endpoints?
Date: Wed, 18 Feb 2009 16:45:46 -0500
From: sbr
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