Just exactly that!
René J.
--- On Thu, 3/12/09, Emily Sours wrote:
From: Emily Sours
Subject: Re: [Histonet] silly question about goat serum
To: histonet@lists.utsouthwestern.edu
Date: Thursday, March 12, 2009, 10:41 AM
What exactly does non-immune goat serum mean? The goat hasn't
Hi Emily,
Normal as opposed to immune serum. If a goat outside of a "Specific
Pathogen Free (SPF)" facility were to actually have no Ab's it would be
far from normal! I'm picturing the Boy in a Bubble episode on Seinfeld-
only substitute one kid for another!! LOL (or groan if you prefer).
Enjoy the
What exactly does non-immune goat serum mean? The goat hasn't been exposed
to any specific antigen, therefore it's normal?
Emily
--
prometheus, thief of light, giver of light, bound by the gods, must have
been a book.
-mark danielewski, house of leaves
Yest, it is.
René J.
--- On Thu, 3/12/09, Eva Permaul wrote:
From: Eva Permaul
Subject: [Histonet] silly question about goat serum
To: histonet@lists.utsouthwestern.edu
Date: Thursday, March 12, 2009, 9:22 AM
Good morning,
I have a question that is probably very silly but I just don't wa
Good morning,
I have a question that is probably very silly but I just don't want to
make any mistakes with the experiment I am suppose to do. I am used to
the protein blocking being called normal goat serum (10%). The kit I am
about to use says their's is 10% goat non-immune serum. Is this the
atomy, UWO
London, Canada
= = =
- Original Message -
From: Susan Bachus
Date: Friday, December 12, 2008 20:33
Subject: Re: [Histonet] Silly Question? - Need help quickly!
To: rjbu...@yahoo.com, Pat Flannery ,
histonet@lists.utsouthwestern.edu, "Weems, Joyce"
> I tr
Check it out, YES formaldehyde fixation is reversible and that is the basis for
HIER.
René J.
--- On Sat, 12/13/08, tf wrote:
From: tf
Subject: Re: RE: [Histonet] Silly Question?
To: "rjbuesa" , "pruegg" , "Merced
Leiker"
Cc: "'histonet@lists.utsout
No.
Once the tisse was fixed, u can never get it fresh again.
2008-12-13
tf
发件人： Rene J Buesa
发送时间： 2008-12-13 05:23:06
收件人： pruegg; Merced Leiker
抄送： 'histonet@lists.utsouthwestern.edu'; 'Pat Flannery'
主题： RE: [Histonet] Silly Question?
Just two things a
I am
trying again with a URL for this paper:
http://www.jneurosci.org/cgi/reprint/13/4/1337
Susan
- Original Message -
From: "Rene J Buesa"
To: "Pat Flannery" ; ;
"Weems, Joyce"
Sent: Thursday, December 11, 2008 12:58 PM
Subject: RE: [Histonet] Silly
ge even by placing them in distilled water instead of HIER (although
it will take much more time).
René J.
--- On Fri, 12/12/08, Merced Leiker wrote:
From: Merced Leiker
Subject: RE: [Histonet] Silly Question?
To: pru...@ihctech.net
Cc: "'histonet@lists.utsouthwestern.edu'" ,
ng because the sample was not adequately fixed.
there i will get off my Friday soap box..
Happy Holidays to all!
Patsy
Original Message
Subject: RE: [Histonet] Silly Question?
From: Merced Leiker
Date: Fri, December 12, 2008 8:12 am
To: "Edwards, R.E.&q
processing because the
sample was = not adequately fixed.
there i will get off my Friday soap box..
Happy Holidays to all!
Patsy
Original Message
Subject: RE: [Histonet] Silly= Question?
From: Merced Leiker
Date: Fri, = December
tonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pat
Flannery Sent: 11 December 2008 16:59
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Silly Question?
Please humor me on this if it's obvious (to everyone but me): why do
we use p
r 2008 16:59
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Silly Question?
Please humor me on this if it's obvious (to everyone but me): why do
we use paraformaldehyde (which is so inconvenient to make up) rather
than buffered formalin or just diluted formaldehyde itself?
It se
ame = methanal or formaldehyde.René
> J.
>
> --- On Thu, 12/11/08, Weems, Joyce wrote:
>
>
> From: Weems, Joyce
> Subject: RE: [Histonet] Silly Question? - Need help quickly!
> To: "Pat Flannery" ,
> histonet@lists.utsouthwestern.edu
> Date: Thursday, Dece
estern.edu] On Behalf Of Pat
Flannery
Sent: Friday, 12 December 2008 4:22 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Silly Question?
Thank you, Jeanine, Joyce, and Linda. The tissues I'm cutting are
just for H&E staining and light microscopy; they won't
at Flannery
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Silly Question?
Hi Pat,
Paraformaldehyde does not contain any additives and is considered more
"pure" than formaldehyde which often contains methanol which in some
cases is undesirable depending on the type of assay
t@lists.utsouthwestern.edu
Subject: [Histonet] Silly Question?
Please humor me on this if it's obvious (to everyone but me): why do
we use paraformaldehyde (which is so inconvenient to make up) rather
than buffered formalin or just diluted formaldehyde itself?
It seems that around here, some fo
Hi Pat:
The differences are largely in the minds of the investigators.
Confusion comes from inexact nomenclature. One part of formaldehyde
(37-40%) plus 9 parts of buffer makes formalin or 10% formalin which is
about 4% formaldehyde. Yes, the 37-40% formaldehyde you buy has some
methanol a
, Joyce
Subject: RE: [Histonet] Silly Question? - Need help quickly!
To: "Pat Flannery" , histonet@lists.utsouthwestern.edu
Date: Thursday, December 11, 2008, 12:12 PM
I was just going to post a question regarding paraformaldhyde myself!
Just last week I believe I remember someone sa
me and sometimes the whole issue
boils down to personal preferences.
René J.
--- On Thu, 12/11/08, Pat Flannery wrote:
From: Pat Flannery
Subject: [Histonet] Silly Question?
To: histonet@lists.utsouthwestern.edu
Date: Thursday, December 11, 2008, 11:58 AM
Please humor me on this if it's ob
:
From: Weems, Joyce
Subject: RE: [Histonet] Silly Question? - Need help quickly!
To: "Pat Flannery" , histonet@lists.utsouthwestern.edu
Date: Thursday, December 11, 2008, 12:12 PM
I was just going to post a question regarding paraformaldhyde myself!
Just last week I believe I remember some
Thank you, Jeanine, Joyce, and Linda. The tissues I'm cutting are
just for H&E staining and light microscopy; they won't be used for
Immunostaining, antigen retrieval, or anything too fancy. I don't see
what difference it would make, but of course I'll put them in whatever
people ask for.
7831 - Fax
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Pat
Flannery
Sent: Thursday, December 11, 2008 11:59 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Silly Question?
Please humor me on this if it's obvious (to everyone but me):
Hi Pat,
Paraformaldehyde does not contain any additives and is considered more
"pure" than formaldehyde which often contains methanol which in some
cases is undesirable depending on the type of assay being conducted.
Linda
Pat Flannery wrote:
Please humor me on this if it's obvious (to ever
Please humor me on this if it's obvious (to everyone but me): why do
we use paraformaldehyde (which is so inconvenient to make up) rather
than buffered formalin or just diluted formaldehyde itself?
It seems that around here, some folks prefer paraformaldehyde (either
2% or 4%) and others u
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