net@lists.utsouthwestern.edu>
Sent: Wednesday, 29 March 2023 4:51 AM
To:
Histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Sudanblack B on FFPET
>
> Gudrun Lang in Austria asks:
>
>>Has anyone experience with Sudanblack B on paraffin slid
onet
Sent: March 28, 2023 4:12 PM
To: Tony Henwood
Cc: Histonet@lists.utsouthwestern.edu ; Bob
Richmond
Subject: Re: [Histonet] Sudanblack B on FFPET
Sudan Black staining won’t work on FFPET. The alcohols and xylenes used in
the tissue processing dissolves the lipids in the tissue. However, you can
> From: Bob Richmond via Histonet<mailto:histonet@lists.utsouthwestern.edu>
> Sent: Wednesday, 29 March 2023 4:51 AM
> To: Histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Sudanblack B on FFPET
>
> >
> > Gudrun La
;
Sent: Wednesday, 29 March 2023 4:51 AM
To: Histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Sudanblack B on FFPET
>
> Gudrun Lang in Austria asks:
>
>>Has anyone experience with Sudanblack B on paraffin slides for staining
[
>
> Gudrun Lang in Austria asks:
>
>>Has anyone experience with Sudanblack B on paraffin slides for staining
[lipofuscin]? A doctor wants the demonstration of the lipoid content of
foamy cells or granulocytes in lung. I've found protocols that have
incubation-times from 10 minutes to over-night.
of Sudan black B certified by the Biological Stain Commission.
John Kiernan
London, Canada
= = =
From: Gudrun Lang via Histonet
Sent: March 28, 2023 10:30 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Sudanblack B on FFPET
Hallo!
Has anyone
Hallo!
Has anyone experience with Sudanblack B on paraffin slides for staining
lipofuszin? A doctor wants the demonstration of the lipoid content of foamy
cells or granulocytes in lung.
I've found protocols that have incubation-times from 10 minutes to
over-night.
I've made a saturated
