Hi Heather,
I can see why you’re having trouble. 30 micron sections are inherently
unstable. Like paraffin, the thicker a section is the more difficult it is to
cut. Plus since your practice samples are old they tend to be more brittle.
Try cutting at 5 microns and see what happens. Remember
Hi Paula,
I am cutting at -24, but have tried going as warm as -18. I am
currently learning with 30uM sections with the ultimate goal of moving
towards 5 or 10uM. Our lab standard has been collecting into millonigs
solution and doing free floating IHC. We generally have no issue with
this tech
Good Morning Heather,
I have some questions about how you cut frozen brains.
What temperature are you cutting at?
How thick are your sections?
How are your samples frozen? Flash freezing, slow freezing, iso-pentane in
LN2?
Your answers may provide clues to help you get better cryosections
Hello Histonet,
I'm looking into working up a tape transfer method of collecting
cryosections of brain while preserving infarct to be used in IHC. I
find that when I try and section heavily damaged regions of the brain
the tissue tears and and I lose it. Has anyone got any recommendations
about
