Subject: [Histonet] Tris Buffer Preservative
Hi,
I have been making up my own tris buffer for immunohistochemistry. It has
been working swimmingly, with one exception. I have been doing some work on
Salmonella, and apparently the researcher has been seeing bacteria in her
Hi,
I have been making up my own tris buffer for immunohistochemistry. It has
been working swimmingly, with one exception. I have been doing some work on
Salmonella, and apparently the researcher has been seeing bacteria in her
immunofluorescent slides that she says are moving and seem to be
I never used preservatives but I also never stored the buffer more than 5 days.
René J.
--- On Wed, 2/24/10, Amos Brooks amosbro...@gmail.com wrote:
From: Amos Brooks amosbro...@gmail.com
Subject: [Histonet] Tris Buffer Preservative
To: histonet@lists.utsouthwestern.edu histonet
Hello, Amos,
It is pretty straightforward to make fresh, why would you want to
store it? We use sterile 1M Tris, pH 7.4, and 4M NaCl as stocks, they
are concentrated enough that nothing obvious grows in them, dilute
the volume you need, pH if needed, (add the Tween if needed), and
store in
