Hi Everyone!
I have seen unstained slides save a patient from re-biopsy many times.
Usually it will be a case where a patient has a known diagnosis, like lung
cancer. In these types of cases after diagnosis molecular testing (and IHC
for PD-L1) is usually ordered. There have been countless
To expand on what we do at our research lab; we use 6 months as a standard
maximum age of unstained slides. Also in the staining protocol for each
antibodyl we have a specific shelf life for the diluted Antibody and a
maximum age of unstained slides.
Jamie
I agree with Jamie
Only a few Ags are "oxidised" ( that's the term used, I recall) but, don't let
it be YOUR protein of interest.
If you really are concerned, cut fresh sections and immunostain along with your
stored sections.
Imho: cut as few sections as you need.
Store any unused at 4C
I’m a histology workflow consultant that visits many AP laboratories
each year. Almost every laboratory has a different retention policy.
The average of most laboratories is to hold onto unstained slides for
three weeks after final sign out. Typically the unstained slide can be
held for a long
a Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, August 16, 2018 4:49 PM
To: HistoNet
Subject: [Histonet] Unstained slides - how long are they good for?
Hello My Fellow Histologists,
Happy Friday Eve.
The question has come up.. How long are *unstained* slides good for?
nohistochemistry & Molecular Morphology, 26(7), 489-494.
-Original Message-
From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, 17 August 2018 9:49 AM
To: HistoNet
Subject: [Histonet] Unstained slides - how long are they good for?
Hello My Fellow Histo
It depends on the stability of the protein and fixation, some are stable
for a week some for years. We use 6 months as a standard.
Jamie
On August 16, 2018 4:59:34 PM P Sicurello via Histonet
wrote:
> Hello My Fellow Histologists,
>
> Happy Friday Eve.
>
> The question has come up.. How
Hello My Fellow Histologists,
Happy Friday Eve.
The question has come up.. How long are *unstained* slides good for?
Not for H but tests like IHC and molecular testing. These slides have
been cut, stored at room temperature, not sealed in anyway, and kept in a
cardboard box.
Please let me
