[Histonet] What is 10% Buffered Formalin Acetate

2011-07-28 Thread Harvey, Jennifer Lynn
I had a investigator say that he is using 10% Buffered Formalin Acetate to fix his mouse brains. They order it from Fisher (127-09-3). It is buffered with Sodium Acetate instead. Has anyone ever used this and why would you want to? I have never heard of it before! It is a new one to me!

Re: [Histonet] What is 10% Buffered Formalin Acetate

2011-07-28 Thread Geoff McAuliffe
More likely buffered with calcium acetate. The late Ralph Lillie advocated 2% calcium acetate as a good buffer for formalin mixtures. One gets buffering plus some calcium ions to help with the stabilization of lipids.Also easy, just one weighing. Anything Lillie recommends is on a very sound