For formaldehyde-fixed material the pH has to be lower than for methanol-fixed
blood or marrow smears (for which pH6.8 is best).
For your sections try pH 5.0 first. Go down in steps of 0.5 if too blue; up in
steps of 0.5 if too red. The colour scheme is not a varied as with a blood
film, but
Hi all,
I am trying to look at eosinophils and neutrophils in FFPE muscle sections.
Does anyone have experience with the Wright stain or a Wright staining kit on
these types of sections? Or possibly a suggestion for a different protocol to
visualize these cells?
Thanks!
Becky
, 2014 3:18 PM
Subject: [Histonet] Wright Stain on paraffin sections
Hi all,
I am trying to look at eosinophils and neutrophils in FFPE muscle sections.
Does anyone have experience with the Wright stain or a Wright staining kit on
these types of sections? Or possibly a suggestion for a different
