ve to use an anti beta Gal antibody.
> Finding an anti beta Gal enzyme antibody, that works in FFPWS +/- AR is
> not so easy.
>
> Good luck
>
> Carl
>
> Carl Hobbs FIBMS
> Histology and Imaging Manager
> Wolfson CARD
> Guys Campus, London Bridge?
> Kings College London
&
I agree with Carl about the X-gal activity being destroyed by processing
for paraffin sections (I'm a researcher, not a histologist).
However, in our hands the anti-b-galactosidase antibody, reference
A-11132, from ThermoFisher DOES work on paraffin sections (many others
don't). It has been pu
Naira)
2. (no subject) (Waitts, Celeste)
--
Message: 1
Date: Tue, 7 Jan 2020 18:21:31 +
From: "Margaryan, Naira"
To: "'histonet-request (histonet@lists.utsouthwestern.edu)'"
Subje
Tue, 7 Jan 2020 18:21:31 +
From: "Margaryan, Naira"
To: "'histonet-request (histonet@lists.utsouthwestern.edu)'"
Subject: [Histonet] X-gal staining
Message-ID:
Content-Type: text/plain; charset="us-ascii"
Hello histonetters
Hello histonetters,
I have request from a scientist to perform X-gal staining on the formalin fixed
paraffin-embedded kidney.
I was wondering if there is any possibility to do this staining on FFPE tissue
as well as if there any possibility to perform a peroxidase DAB staining for
the X-gal? I
opher Kalleberg"
To: histonet-requ...@lists.utsouthwestern.edu,
histonet@lists.utsouthwestern.edu
Sent: Thursday, January 8, 2015 9:53:38 AM
Subject: [Histonet] X-gal staining
Hello All,
Does anyone know of a commercially available X-gal stain that would work on
formalin fixed paraffi
Hello All,
Does anyone know of a commercially available X-gal stain that would work on
formalin fixed paraffin embedded skin samples in order to detect beta
galactosidase. Thank you in advance.
Kris
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Histonet@lists.utsouthwes
Hi all,
I have a question about whole mount x-gal staining. Do any of you have
experience with your samples turning brown after incubation with the x-gal? If
yes, do you know what causes this and how to keep it from happening?
Thanks in advance,
Teri Johnson, HT(ASCP)QIHC
Managing Director, H
ther factors can cause false positive
and false negative X-gal staining. Do your homework!
John Kiernan
Anatomy, UWO
London, Canada
= = =
- Original Message -
From: Nicholas David Evans
Date: Thursday, August 13, 2009 15:51
Subject: [Histonet] X-gal staining
To: hist
Hello,
I was wondering whether anyone might advise me on X-gal staining.
I am looking at the expression of LacZ knocked into the locus of our gene
of interest. We cryoembed, take sections and stain for beta-galactosidase
activity using X-gal. The blue stain is often punctuate and noticeably
limi
...@lists.utsouthwestern.edu
To: histonet@lists.utsouthwestern.edu
Sent: Jan 13, 2009 11:25 AM
Subject: [Histonet] X-Gal staining
I am interested in any suggestions for what mouse tissue organ and route
of administration that I could use as a postitive x-gal staining control
to tease out tissue
I am interested in any suggestions for what mouse tissue organ and route
of administration that I could use as a postitive x-gal staining control
to tease out tissue processing, staining and paraffin embedding
artifacts.
Regards,
MH
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