RE: [Histonet] Xylene Substitute for Counterstain Clearing

2012-05-11 Thread gayle callis
, Andrew Coleman wrote: From: Andrew Coleman Subject: [Histonet] Xylene Substitute for Counterstain Clearing To: histonet@lists.utsouthwestern.edu Date: Friday, May 11, 2012, 12:25 PM Hi all, We are performing a neutral red counterstain on tissue sections containing colored polystyrene

Re: [Histonet] Xylene Substitute for Counterstain Clearing

2012-05-11 Thread Rene J Buesa
Coleman Subject: [Histonet] Xylene Substitute for Counterstain Clearing To: histonet@lists.utsouthwestern.edu Date: Friday, May 11, 2012, 12:25 PM Hi all, We are performing a neutral red counterstain on tissue sections containing colored polystyrene microspheres. The spheres are inert to alcohol

Re: [Histonet] Xylene Substitute for Counterstain Clearing

2012-05-11 Thread Victoria Baker
I think that it is called Crystal Mount - but apply to section, allow to harden dip slide in clearing media and coverslip. I know that there must be others out there as well. Good Luck On Fri, May 11, 2012 at 12:25 PM, Andrew Coleman wrote: > Hi all, > > We are performing a neutral red counte

RE: [Histonet] Xylene Substitute for Counterstain Clearing

2012-05-11 Thread Cynthia Pyse
-Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrew Coleman Sent: Friday, May 11, 2012 12:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitute for Counterstain Clearing Hi all, We are

RE: [Histonet] Xylene Substitute for Counterstain Clearing

2012-05-11 Thread Elizabeth Chlipala
] On Behalf Of Andrew Coleman Sent: Friday, May 11, 2012 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitute for Counterstain Clearing Hi all, We are performing a neutral red counterstain on tissue sections containing colored polystyrene microspheres. The spheres

[Histonet] Xylene Substitute for Counterstain Clearing

2012-05-11 Thread Andrew Coleman
Hi all, We are performing a neutral red counterstain on tissue sections containing colored polystyrene microspheres. The spheres are inert to alcohol, but are washed out when we clear with xylene to coverslip. The spheres are also supposedly soluble in DMF, acetone, acetonitrile, chloroform and me