RE: [Histonet] air drying special stain slides rather than dehydrate and clear

2012-09-12 Thread Horn, Hazel V
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Tuesday, September 11, 2012 7:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] air drying special stain slides rather than dehydrate and clear Hi, My choice to air dry rather than dehydrate in ETOH

Re: [Histonet] air drying special stain slides rather than dehydrate and clear

2012-09-12 Thread Rene J Buesa
: [Histonet] air drying special stain slides rather than dehydrate and clear I suppose my age will show here but I was always taught to NEVER let slides dry out unless the procedure indicated such.  Is there no drying artifact when you let these slide dry before coverslipping? Hazel Horn Supervisor

RE: [Histonet] air drying special stain slides rather than dehydrate and clear

2012-09-12 Thread Amos Brooks
...@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Tuesday, September 11, 2012 7:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] air drying special stain slides rather than dehydrate and clear Hi, My choice to air dry rather than dehydrate in ETOH xylene is based

[Histonet] air drying special stain slides rather than dehydrate and clear

2012-09-11 Thread Diana McCaig
I was hoping to get information on why special stains are dehydrated, cleared and mounted vs allowing them to be blotted dry, air dried then coverslip. Every procedure I have ever encountered always indicates to dehydrate and clear but I have heard where some labs are blotting the slides ,

[Histonet] air drying special stain slides rather than dehydrate and clear

2012-09-11 Thread Amos Brooks
-0400 From: Diana McCaig dmcc...@ckha.on.ca Subject: [Histonet] air drying special stain slides rather than dehydrate and clear To: histonet@lists.utsouthwestern.edu Message-ID: dcfd9e6a390e294aaf3a2561cd32e5c417a90...@ckhamail1.ckha.on.ca Content-Type: text/plain