- Original Message -
From: e...@pigsqq.org [mailto:e...@pigsqq.org]
Sent: Tuesday, August 21, 2012 05:46 AM
To: Debra Siena
Subject: Re: [Histonet] annoying crystals on sections
We just now ran the statlab version protocol from StatLab's website, using an
alcoholic eosin. No doubt
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www.statlab.com
- Original Message -
From: e...@pigsqq.org [mailto:e...@pigsqq.org]
Sent: Tuesday, August 21, 2012 05:46 AM
To: Debra Siena
Subject: Re: [Histonet] annoying crystals on sections
We just now ran the statlab version protocol from StatLab's website, using an
alcoholic eosin
Obviously, it's your water, if you don't see the crystals after
changing your water. If the acidic pH of the Milli-q water is leaching out
your eosin, then adjust the pH of the water to 7.0 before using it to rinse
your slides.
Sincerely,
We are having problems with crystals precipitated on our slides which
are HE stains on tissues from pigs.
Tissues are fixed in buffered formalin. We had trouble months ago with
formalin pigment and we had resolved
that by using ammonia in EtOH or picric acid in EtOH. Sometimes we
receive
