Are your PAP smear slides fixed in alcohol? If so, you probably don't need
antigen retrieval.
Richard
Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596
(860) 545-0174 Fax
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of hao
zhang
Sent: Sunday, 14 September 2008 1:12 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] antigen retrieval for
[Histonet] antigen retrieval for 5 year pap smear slides
Hi, everyone
I want to do IHC from a 5 year old pap smear slides for pan-keratin
stain.
I tried many methods of antigen retrieval protocols (10 mM citric
buffer, ph 6; 1 mM EDTA, Ph8; 10 mM Tris buffer, Ph 10; ) and heat at
95C for 40 minute
net] antigen retrieval for 5 year pap smear slides
To: histonet@lists.utsouthwestern.edu
Date: Saturday, September 13, 2008, 11:11 PM
Hi, everyone
I want to do IHC from a 5 year old pap smear slides for pan-keratin stain.
I tried many methods of antigen retrieval protocols (10 mM citric buffer
Hi, everyone
I want to do IHC from a 5 year old pap smear slides for pan-keratin stain.
I tried many methods of antigen retrieval protocols (10 mM citric buffer, ph
6; 1 mM EDTA, Ph8; 10 mM Tris buffer, Ph 10; ) and heat at 95C for 40
minutes. I also used Triton x 100 (0.2%) pretreatment 10 minut
