Re: [Histonet] antigen retrieval for 5 year pap smear slides

2008-09-15 Thread Richard Cartun
Are your PAP smear slides fixed in alcohol? If so, you probably don't need antigen retrieval. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax

RE: [Histonet] antigen retrieval for 5 year pap smear slides

2008-09-14 Thread Tony Henwood
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of hao zhang Sent: Sunday, 14 September 2008 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] antigen retrieval for

RE: [Histonet] antigen retrieval for 5 year pap smear slides

2008-09-14 Thread Tony Henwood
[Histonet] antigen retrieval for 5 year pap smear slides Hi, everyone I want to do IHC from a 5 year old pap smear slides for pan-keratin stain. I tried many methods of antigen retrieval protocols (10 mM citric buffer, ph 6; 1 mM EDTA, Ph8; 10 mM Tris buffer, Ph 10; ) and heat at 95C for 40 minute

Re: [Histonet] antigen retrieval for 5 year pap smear slides

2008-09-14 Thread Rene J Buesa
net] antigen retrieval for 5 year pap smear slides To: histonet@lists.utsouthwestern.edu Date: Saturday, September 13, 2008, 11:11 PM Hi, everyone I want to do IHC from a 5 year old pap smear slides for pan-keratin stain. I tried many methods of antigen retrieval protocols (10 mM citric buffer

[Histonet] antigen retrieval for 5 year pap smear slides

2008-09-13 Thread hao zhang
Hi, everyone I want to do IHC from a 5 year old pap smear slides for pan-keratin stain. I tried many methods of antigen retrieval protocols (10 mM citric buffer, ph 6; 1 mM EDTA, Ph8; 10 mM Tris buffer, Ph 10; ) and heat at 95C for 40 minutes. I also used Triton x 100 (0.2%) pretreatment 10 minut