first and block the peroxidase afterwards.
On the other hand, many labs do
René J.
--- On Thu, 3/1/12, Tuyen Nguyen wrote:
From: Tuyen Nguyen
Subject: [Histonet] blocking endogenous activities
To: histonet@lists.utsouthwestern.edu
Date: Thursday, March 1, 2012, 6:48 PM
In my lab, in IHC
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu [mailto:
> histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tuyen Nguyen
> Sent: Thursday, March 01, 2012 3:49 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] blocking endogenous
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tuyen Nguyen
Sent: Thursday, March 01, 2012 3:49 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] blocking endogenous activities
In my lab, in IHC protocols
In my lab, in IHC protocols for most antibodies, hydrogen peroxide is applied
before antigen heat retrieval. However, I wonder there is any different result
in IHC stain if hydrogen peroxide for blocking endogenous activities is applied
after antigen heat retrieval by using steamer or pressure c
