RE: [Histonet] fish eggs: processing and fixation

2014-10-23 Thread Thompson, Christy
Sadly we don't have the capacity to do frozen sections :( Christy -Original Message- From: Diane G. Miller [mailto:dgmil...@coho.net] Sent: October 23, 2014 11:56 AM To: Thompson, Christy Subject: Re: [Histonet] fish eggs: processing and fixation Have you tried doing frozen sec

Re: [Histonet] fish eggs: processing and fixation

2014-10-23 Thread Jay Lundgren
Usually fish eggs are only processed by lightly salting and pressing. I've never tried sectioning them. Just serve with toast points and lemon wedges, maybe a little minced onion, and of course dry champagne! Sincerely?

[Histonet] fish eggs: processing and fixation

2014-10-23 Thread Thompson, Christy
Hi all, I'm currently working on some unfertilized fish eggs. They were fixed in 10% NBF and processed in a vacuum processor with paraffin. The eggs are extremely hard to section and most of the yolk goes AWOL on the way to the water bath. I did do a search here for some help and a few things were