I will 2nd this.
When I did neuropathology at a major institution, we froze all frozen sections
in an isopentane slurry cooled with LN2. We waited for the OCT to warm to
cryostate temps before cutting. If there was time pressure from the surgeons, I
used my thumb to warm more quickly, until se
There is an OCT for lower temperatures.
- Original Message -
From: "Della Speranza, Vinnie"
To: "Bruce W Brodersen" ,
histo...@pathology.swmed.edu
Sent: Monday, August 9, 2010 9:40:03 AM
Subject: RE: [Histonet] friable or crumbly O.C.T.
I'm guessing that l
26 PM
To: histo...@pathology.swmed.edu
Subject: [Histonet] friable or crumbly O.C.T.
Anyone have an explanation as to why OCT would be friable or crumbly for
sectioning? Here's how it was used.
Thanks.
"We held the plastic 'tray' with the tissue in the compound just over the
liquid
Anyone have an explanation as to why OCT would be friable or crumbly for
sectioning? Here's how it was used.
Thanks.
"We held the plastic 'tray' with the tissue in the compound just over the
liquid nitro for 30sec-1min, until it was opaque and white (frozen) and
then dipped the tray into the li
