I can't speak to the the T-cell receptor sites but the only option for
getting decent sections at this point is option number two. In the future,
(if there applicable) the cut surface of a cryosectioned block can be
recovered with OCT, frozen, and stored in an air-tight whirl-pak or ziplock
bag in
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-Original Message-
From: Terri Braud via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, January 04, 2017 12:16 PM
To: 'histonet@lists.utsouthwestern.edu'
Cc: 'r...@psu.edu'
Subject: Re: [Histonet] Grad Student
My response:
My first question is what temperature are the blocks that are too brittle? And
at what temperature are you trying to cut? Blocks stored in cryofreezers at
-70o C or less are far too cold to cut without brittleness. My suggestion
would be to pull the blocks and put them into the
Completely agree...it sounds like the tissue got freeze dried and I doubt
if any staining you might get after lots of work will be completely
reliable.
Loralei
On Jan 3, 2017 11:02 AM, "Caroline Miller via Histonet" <
histonet@lists.utsouthwestern.edu> wrote:
> I hate to say it but I think
I hate to say it but I think these tissues are toast. It seems they had bad
fixation or handling to start with, and I would question whether the
tissues were left out to dry before fixation or freezing. Or whether they
got fixed and then sucrose infiltrated (which is my method of choice if the
I got the following from a grad student here at Penn State. I am not sure how
to solve his problem if possible. Does anyone have any suggestions I can
forward?
Roberta Horner
Animal Diagnostic Lab
Penn State University
"I am having some difficulties sectioning mouse tumor samples for
