[Histonet] H. pylori

2012-02-14 Thread Rathborne, Toni
Happy Valentines Day! Our lab is looking into ordering H. pylori now and I was wondering what clone, or whose antibody everyone is using. As always, thanks for your responses. I know I can always count on this group to offer opinions and suggestions :) Toni CONFIDENTIALITY NOTICE This messa

[Histonet] H. pylori

2014-03-27 Thread Abbott, Tanya
Can anyone recommend a good commercially bought slide for H.pylori? Thanks! Tanya Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This electronic mail and

[Histonet] H. Pylori

2015-09-18 Thread Nancy Schmitt via Histonet
Good Morning- I am looking for H. Pylori control - does anyone have any extra lying about? What are you currently using? Purchasing? I appreciate any and all feedback! Nancy Schmitt MLT, HT(ASCP) United Clinical Laboratories Dubuque, IA 52001 Check us out at www.uclaccess.com

[Histonet] h. pylori

2016-02-23 Thread Dooley, Elaine via Histonet
Hi, We filter our H.Pylori antibody from Cell Marque before using it on our Ventana Ultra and XTs and this has made it a cleaner stain. Elaine Dooley Shands Teaching Hospital Gainesville FL 352-265-0111 ext 72117 ___ Histonet mailing list Histonet@li

[Histonet] h. pylori

2016-02-26 Thread Dooley, Elaine via Histonet
Hi histonetters, We filter the H.pylori antibody with the filters that come with the Ventana Prep kits. Elaine Dooley Shands Teaching Hospital 352-265-0111 ext 72117 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwester

[Histonet] H. Pylori - IHC

2011-11-16 Thread Beth.Fye
Does anyone have recommendations for a good vendor for H.Pylori for IHC? We have tried many, but our pathologists complain that they are not specific for H.Pylori and that all gram negative bacteria are staining. Currently we are not running this but our pathologists are interested in it. Th

[Histonet] H Pylori Control

2012-01-25 Thread Rae Staskiewicz
Good Afternoon everyone, My institution is about to start running H Pylori for another lab. They microwave process their tissue. Would there be anyone out there who microwave processes and would have one or two blocks of H Pylori positive control tissue they would be willing to let us have?

Re: [Histonet] H. pylori

2012-02-14 Thread Richard Cartun
Keep in mind that many of the commercially-available monoclonal antibodies to H. pylori do not label Helicobacter helilmannii, often seen in children, especially those with exposure to animals. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collec

[Histonet] H Pylori Stain

2013-01-03 Thread Tasha Fraser
Hi everyone! Does anyone have a good h-pylori stain? We currently use the Steiner and Steiner Stain. We've done some testing with the Alcian Yellow-Toluidine Blue Method, but not getting the same results as the book shows. Does anyone have any suggestions please. Tasha Fraser , HT (ASCP) O

[Histonet] H. Pylori Testing

2015-04-27 Thread Vickroy, James
We are a small lab processing mostly GI specimens. Currently we are sending our H. Pylori testing to a local hospital for staining however I can predict that this year we may have around 1000 H. Pylori stains done. I am looking for a small platform or manual test kit to perform the H. Pylori'

[Histonet] H. Pylori Testing

2015-04-29 Thread Amos Brooks
into a catch tray below. It makes it **really** easy to reproducibly do IHC manually. Amos Brooks On Tue, Apr 28, 2015 at 12:24 PM, wrote: > Message: 8 > Date: Mon, 27 Apr 2015 16:49:25 -0400 > From: Garreyf > Subject: Re: [Histonet] H. Pylori Testing > To: Michael Ann Jones

[Histonet] H. pylori stain

2016-09-01 Thread Amy Johnson via Histonet
Hello histoland We currently use Ventana's h. pylori antibody on our Benchmark ultra. Over the last few months our pathologists have been noticing "junk" that is staining with this antibody. It seems to be on the tissue more than in the tissue and its not on all slides. WE have tried changin

[Histonet] H Pylori controls

2021-01-21 Thread Cynthia McGrady via Histonet
Hello Histonetters. Looking for positive H Pylori control block(s). We typically use positive case tissue from old surgical cases, but seem to be running low. If anyone has a surplus, and would be willing to share, or maybe we have tissue you can use?? We could do a swap..Let me know..Thanks...Cind

[Histonet] H Pylori controls

2021-01-22 Thread Hans B Snyder via Histonet
> > Message: 1 > Date: Thu, 21 Jan 2021 13:21:14 -0500 > From: Cynthia McGrady > To: Histonet > Subject: [Histonet] H Pylori controls > Message-ID: > < > cafn2hshezlsrd-8brzj+8ezknn3zxvoglz93+bgmov820sz...@

[Histonet] H Pylori IHC

2021-09-28 Thread Rapp, Keith via Histonet
Hello, we have just purchased the Leica Bond Max IHC stainer and love it. It's easy to use and produces beautiful stains. However, Helico Pylori has been giving us problems with weak and incomplete staining (not all of the organisms staining). Is there anyone using the Bond Max and successfully s

[Histonet] H. PYLORI STAIN

2010-12-01 Thread Rathborne, Toni
Hi, I was wondering if anyone has used the Poly Scientific "Rapid Staining Method for H. Pylori in Gastric Biopsies" staining kit. Is it easy to use? Is the stain crisp and easy to read? Any feedback would be appreciated. Toni CONFIDENTIALITY NOTICE This message and any included attachments a

Re: [Histonet] H. Pylori - IHC

2011-11-16 Thread Drew Meyer
We use the Rabbit Polyclonal H. pylori from Cell Marque on the Ventana Ultra's... We're a natinowide reference laboratory and our clients are all very happy with the specificty of the staining. Let me know if there's any more information you need. Drew On Wed, Nov 16, 2011 at 17:15, wrote: > Do

RE: [Histonet] H. Pylori - IHC

2011-11-16 Thread Sebree Linda A
beth@hcahealthcare.com Sent: Wednesday, November 16, 2011 4:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H. Pylori - IHC Does anyone have recommendations for a good vendor for H.Pylori for IHC? We have tried many, but our pathologists complain that they are not specific for H.P

RE: [Histonet] H Pylori Stain

2013-01-03 Thread joelle weaver
If you want a special stain- How about the good 'ole Giemsa with Jenner's? Works fine, is cheap and quick. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: tfra...@olympicmedical.org > To: histonet@lists.utsouthwestern.edu > Date: Thu, 3 Jan 2013 11:22:46 -0800 > Subjec

Re: [Histonet] H Pylori Stain

2013-01-03 Thread Rene J Buesa
You can find my procedure at http://www.histosearch.com/rene.html René J. From: Tasha Fraser To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, January 3, 2013 2:22 PM Subject: [Histonet] H Pylori Stain Hi everyone!  Does anyone have a good h-pylori stain?  We currently use t

Re: [Histonet] H Pylori Stain

2013-01-04 Thread Katelin Lester
We use the HP Blue stain by Anatech. Katelin Lester, HTL On Jan 3, 2013 11:23 AM, "Tasha Fraser" wrote: > Hi everyone! Does anyone have a good h-pylori stain? We currently use > the Steiner and Steiner Stain. We've done some testing with the Alcian > Yellow-Toluidine Blue Method, but not gett

Re: [Histonet] H. Pylori Testing

2015-04-27 Thread Michael Ann Jones
We recently switched to IHC stain for HP, however, before that we used Giemsa regressively for those. Not as sensitive as IHC. For IHC we used Cell Marque by hand (with great results) and now we are automated. Michael Ann Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr.

Re: [Histonet] H. Pylori Testing

2015-04-27 Thread Garreyf
Is it a pain in the neck to do it by hand? I'd like to bring my h pyloris in house as well. I'm trying to create more revenue to support a 2nd histotech. Garrey Sent from my iPhone > On Apr 27, 2015, at 4:11 PM, Michael Ann Jones wrote: > > We recently switched to IHC stain for HP, however,

Re: [Histonet] H. Pylori Testing

2015-04-27 Thread Michael Ann Jones
If you do it by IHC it is the normal 2 hour or so process step-by-step. Giemsa is quick 15 minutes or around there? Again, not as sensitive but works. Michael Ann On 4/27/15, 2:49 PM, "Garreyf" wrote: >Is it a pain in the neck to do it by hand? I'd like to bring my h >pyloris in house as we

Re: [Histonet] H. Pylori Testing

2015-04-29 Thread Paula Pierce
Wednesday, April 29, 2015 4:48 PM Subject: [Histonet] H. Pylori Testing Hi,     If you are looking at the possibility of doing IHC manually, I would really recommend the Shandon Sequenza. It is a box that holds clips that attach to your slides. The slides are held vertically and you drop rea

[Histonet] H. pylori control tissue

2015-09-18 Thread Cartun, Richard via Histonet
Nancy Schmitt from Dubuque, IA asked about control tissue for H. pylori. I have tried e-mailing her, but my e-mail was returned as "Undeliverable". Nancy, please contact me. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteo

[Histonet] H. pylori by IHC

2016-02-26 Thread Joanne Clark via Histonet
Recently someone placed a query regarding the precipitate with Cell Marques H. pylori marker. The response was to filter it before use to clean it up. May I ask how it is being filtered? We have the same problem and it makes the slide difficult to interpret. Joanne Clark, HT BAAS Director o

[Histonet] H Pylori control blocks

2016-06-28 Thread Mike Pence via Histonet
Hey Histo world- Does anyone have any blocks to spare that are + for H Pylori? I could sure use a couple for IHC. Michael S. Pence | Supervisor of Laboratory Services Great River Health Systems 1221 S. Gear Ave. | West Burlington, IA 52655 Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-455

Re: [Histonet] H. pylori stain

2016-09-02 Thread Duddey, Aimee via Histonet
[mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, September 01, 2016 3:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H. pylori stain Hello histoland We currently use Ventana's h. pylori antibody on our Benchmark ultra. Over the last few months our pathologists have been not

Re: [Histonet] H Pylori controls

2021-01-22 Thread Mca Werdler via Histonet
ecian) > >4. water under sectios (Bernice Frederick) > > > > > > -- > > > > Message: 1 > > Date: Thu, 21 Jan 2021 13:21:14 -0500 > > From: Cynthia McGrady > > To: Histonet > >

[Histonet] H pylori by IHC

2010-03-19 Thread Kelley Durden
Does anyone have a recommendation for learning IHC for H pylori?   Nice product? Nice protocol? Nice learning tool? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] H. pylori RTU antibody

2010-06-30 Thread Drew Meyer
I was just wondering if a lot of people use the RTU antibodies available out there for H. pylori. Currently, we're testing the RTU from Dako and we're having issues with a lot of background staining. After tweaking the protocol a lot, I've managed to get a lot of it removed, but there are still s

[Histonet] H pylori immuno kit

2009-02-04 Thread Rutledge, Nancy
Looking for a company that sells simple, all inclusive H pylori immunoperoxidase kit to be done manually. Please reply directly to pmea...@capecodhealth.org Vendors welcome. Thank you. Nanacy Rutledge Falmouth Hospital Falmouth, MA 02540 ~~~

[Histonet] H pylori and Ventana instruments

2013-05-07 Thread Rae Staskiewicz
Hi all, Those of you using Ventana instrumentation, could you tell me what H pylori antibody you are using, and whether or not you are experiencing non-specific staining, or staining that looks specific but with a stippled effect. Thanks Rae Staskiewicz UnityPoint Health-Methodist _

Re[2]: [Histonet] H. Pylori Testing

2015-04-28 Thread Brendal Finlay
ate: 04/27/2015 15:49 Subject: Re: [Histonet] H. Pylori Testing Is it a pain in the neck to do it  by hand? I'd like to bring my h pyloris in house as well. I'm trying to create more revenue to support a 2nd histotech. Garrey Sent from my iPhone > On Apr 27, 2015, at 4:11 PM,

RE: [Histonet] H pylori by IHC

2010-03-19 Thread Sebree Linda A
.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kelley Durden Sent: Friday, March 19, 2010 9:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H pylori by IHC Does anyone have a recommendation for learning IHC for H pylori?   Nice product? Nice protocol? Nice learning

RE: [Histonet] H. pylori RTU antibody

2010-06-30 Thread McMahon, Loralee A
...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Drew Meyer [41dm...@gmail.com] Sent: Wednesday, June 30, 2010 10:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H. pylori RTU antibody I was just wondering if a lot of people use the RTU antibodies

Re: [Histonet] H. pylori RTU antibody

2010-06-30 Thread Mark Tarango
uthwestern.edu] On Behalf Of Drew Meyer [ > 41dm...@gmail.com] > Sent: Wednesday, June 30, 2010 10:41 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] H. pylori RTU antibody > > I was just wondering if a lot of people use the RTU antibodies available > out > t

RE: [Histonet] H pylori and Ventana instruments

2013-05-07 Thread Sebree Linda A
:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rae Staskiewicz Sent: Tuesday, May 07, 2013 12:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H pylori and Ventana instruments Hi all, Those of you using Ventana instrumentation, could you tell me what H pylori antibody you

Re: [Histonet] H pylori and Ventana instruments

2013-05-08 Thread Roger Heyna
We use Ventana's HP antibody, and we do see some of the small, non-specific globs of stain, but the pathologists are comfortable differentiating between the true and false staining since they are familiar with this artefact. Roger Heyna >>> "Rae Staskiewicz" 5/7/2013 12:56 PM >>> Hi all, Tho

RE: [Histonet] H pylori and Ventana instruments

2013-05-08 Thread Truscott, Tom
Sent: Wednesday, May 08, 2013 8:41 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] H pylori and Ventana instruments We use Ventana's HP antibody, and we do see some of the small, non-specific globs of stain, but the pathologists are comfortable differentiating between the true

[Histonet] H. pylori on Rhesus macaque tissue

2010-03-11 Thread Zerfas, Patricia (NIH/OD/ORS) [E]
Dear List servers, Did anyone try this antibody on Rhesus macaque tissue? Do you have a protocol? How were the results? Thanks, Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068

[Histonet] H. pylori on Rhesus macaque tissue

2010-03-11 Thread Amos Brooks
s B on non human tissue all the time and it works fine. Good luck, (and let me know for sure how it goes) Amos Brooks Message: 9 Date: Thu, 11 Mar 2010 11:25:16 -0500 From: "Zerfas, Patricia (NIH/OD/ORS) [E]" Subject: [Histonet] H. pylori on Rhesus macaque tissue To: "'his

[Histonet] H pylori control on ventana immuno stainer

2010-11-21 Thread Gloria Cole
Hi, I am setting up a new lab and I am using the Ventana immuno stainer, does anyone know of any good HP controls I can purchase for now that will work well with the Ventana? Thanks, Gloria ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] H pylori control on ventana immuno stainer

2010-11-22 Thread Mark Tarango
Hi Gloria, Are you still looking for some good HP controls? I could send you a block of one of our controls if you're intersted. I have blocks from several different specimens. Just let me know your address and fedex number. Thanks, Mark On Sun, Nov 21, 2010 at 12:30 PM, Gloria Cole wrote:

RE: SPAM-LOW: [Histonet] H. pylori on Rhesus macaque tissue

2010-03-13 Thread Patsy Ruegg
body binds with. I do Hepatitis B on non human tissue all the time and it works fine. Good luck, (and let me know for sure how it goes) Amos Brooks Message: 9 Date: Thu, 11 Mar 2010 11:25:16 -0500 From: "Zerfas, Patricia (NIH/OD/ORS) [E]" Subject: [Histonet] H. pylori on Rhes