Re: [Histonet] help ! paraffin section

2012-08-08 Thread Rene J Buesa
11:45 PM Subject: [Histonet] help ! paraffin section Hi All I am trying to section adult mouse intestine and skin using paraffin embedding. However, when i section, the tissue is torn although the rest of the paraffin looks perfect. Please suggest why this is happening. Also, sometimes the skin

Re: [Histonet] help ! paraffin section

2012-08-08 Thread Mehmet Fatih BOZKURT
In addition to Rene's comment,to cut coagulated tissue (skin that have new wound crust) and calcified tissue is difficult. On Wed, Aug 8, 2012 at 6:45 AM, Megha Kumar meg...@g.clemson.edu wrote: Hi All I am trying to section adult mouse intestine and skin using paraffin embedding.

[Histonet] help ! paraffin section

2012-08-07 Thread Megha Kumar
Hi All I am trying to section adult mouse intestine and skin using paraffin embedding. However, when i section, the tissue is torn although the rest of the paraffin looks perfect. Please suggest why this is happening. Also, sometimes the skin sections fall off the slides when I perform in situ