Having problems with IHC slides not looking the same between runs. Does it have
to do with how long and at what temp they are in the oven for?
Courtney Pierce
IHC Specialist
Quintiles
Translational RD - Oncology
Innovation
Navigating the new health
610 Oakmont Lane
Westmont, IL 60559
Office: +
Not really. Time in the oven and temp is usually not an issue as long as the
tissue is well fixed/processed/infiltrated.
René J.
--- On Mon, 3/12/12, Courtney Pierce courtney.pie...@quintiles.com wrote:
From: Courtney Pierce courtney.pie...@quintiles.com
Subject: [Histonet] IHC Slides
So...I have run down slides and done antigen retrieval on my FFPE
slides. They are currently in antigen retrieval that has come to room
temperature. I am not going to be able to finish the IHC stains until
tomorrow. Will it be better to keep the slides in the antigen retrieval
solution at 4
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
sgoe...@mirnarx.com
Sent: Wednesday, March 02, 2011 1:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC slides
So...I have run down slides and done antigen retrieval on my FFPE
slides. They are currently
You can put them %3 H2O2 for 10 minutes. and then drop primary antibody and
incubate at 4 degrees overnight.
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Just hold them in buffer overnight.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
sgoe...@mirnarx.com
Sent: Wednesday, March 02, 2011 1:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC
In buffer at 4ºC overnight
René J.
--- On Wed, 3/2/11, sgoe...@mirnarx.com sgoe...@mirnarx.com wrote:
From: sgoe...@mirnarx.com sgoe...@mirnarx.com
Subject: [Histonet] IHC slides
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, March 2, 2011, 2:24 PM
So...I have run down slides and done
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse
Sent: Monday, January 24, 2011 7:58 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] ihc slides
Happy Monday Histonetters
I need to pick the brains of the IHC experts out there in histoland. I run
IHC on the Dako
Cynthia,
Hematoxylin will inactivate the peroxidase (link) in the tissues. You must go
back and re-do the link step and hopefully the antigenic sites will still
accept more streptavidin (or similar) conjugated antibody.
Cheer.
Greg
Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept.
I should add that the DAB step may also have quenched all of the linked
peroxidases even before the hematoxilin was added (which would finish off any
that remained).
Greg
Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box
Hi Cynthia,
I agree with Greg, you have to re-do your staining from beginning (from AR
step) after removing the hematoxilin.
Naira
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Tony's answer (62 degrees C for 25 mins) is the minimum to ensure section
adheres to slide. We all have to stain slides that have been baked longer than
30 mins. However, especially for Her2, longer baking times can result in false
negatives or at least diminished staining. The justification
, AUSTRALIA
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gagnon,
Eric
Sent: Thursday, 22 April 2010 5:31 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC Slides, Hotplate vs Oven
Tony's
I'm curious as to whether or not others put their IHC slides on a
hotplate after cutting? If so, at what temperature and for how long?
Who only uses an oven - and what temp and for how long? Does anyone use
both the hotplate and the oven?
Laurie Colbert
We use an oven only at 60c for 20 minutes.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie
Colbert
Sent: Tuesday, April 20, 2010 9:40 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC
@lists.utsouthwestern.edu
Subject: [Histonet] IHC Slides, Hotplate vs Oven
I'm curious as to whether or not others put their IHC slides on a
hotplate after cutting? If so, at what temperature and for how long?
Who only uses an oven - and what temp and for how long? Does anyone use
both the hotplate
: Wednesday, 21 April 2010 12:40 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC Slides, Hotplate vs Oven
I'm curious as to whether or not others put their IHC slides on a hotplate
after cutting? If so, at what temperature and for how long? Who only uses an
oven - and what temp
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