Re: [Histonet] microtome blades and wax

2022-01-14 Thread Rathborne, Toni via Histonet
and the attachments, if any, and destroy any printouts. -Original Message- From: Piche, Jessica via Histonet Sent: Friday, January 14, 2022 8:43 AM To: Histonet Subject: [Histonet] microtome blades and wax *** This is an External Email *** Good Morning, We have been using Leica

Re: [Histonet] microtome blades and wax

2022-01-14 Thread P Sicurello via Histonet
Hi Jessica, Happy Friday. Do you use the same EM-400 paraffin for processing? If you use different paraffins that might contribute to your sectioning problems. If the waxes are different the tissue and the rest of the block are not one continuous unit in the "eyes" of the knife. The

[Histonet] microtome blades and wax

2022-01-14 Thread Piche, Jessica via Histonet
Good Morning, We have been using Leica Histocore Autocut microtomes for a few months and have been having inconsistency with cutting on them. We have tried so many different blades, high and low, and have tried changing the blade angle. Nothing is a perfect fix, and we feel like we are really