Re: [Histonet] P16

You might check Cancer Diagnostics, inc.. Their rep told us they have an IVD p16. Best, Mike -Original Message- From: Charles Riley via Histonet Sent: Wednesday, March 13, 2019 1:24 PM To: Bernice Frederick Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] P16 We

[Histonet] p16

2019 16:11:09 + From: Bernice Frederick To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] P16 Who is the best and current supplier for p16? We were using biocare,but evidently it is no longer available from them. And no Santa Cruz per my IHC tech. Thanks, Bernic

Re: [Histonet] P16

We by predilute from BioGenix On Wed, Mar 13, 2019 at 12:27 PM Bernice Frederick via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Who is the best and current supplier for p16? We were using biocare,but > evidently it is no longer available from them. And no Santa Cruz per my IHC >

[Histonet] P16

Who is the best and current supplier for p16? We were using biocare,but evidently it is no longer available from them. And no Santa Cruz per my IHC tech. Thanks, Bernice Bernice Frederick Pathology Core Facility Robert H. Lurie Cancer Center 710 North Fairbanks Court Olson 8-421 312-503-3723

[Histonet] p16 and sperm morphology

Hello- 1. We are currently running P16 on the Leica Bond Max - are you running or reporting this any differently now that they are 510k FDA approved? 2. Sperm Morphology- are your cytotechnologists reading and reporting out sperm morphology or are your clinical lab techs?

[Histonet] p16 dilution

Hi Histonetters, Does any of you use p16 Cintec from Roche/Ventana? We wanted to dilute this antibody but wasn't sure how much to dilute it to. Figured we could save a lot by doing so. We have been staining our p16 with no current dilution. Any suggestions will help. If there are any other

Re: [Histonet] p16 Antibody

Goodwin Anatomic Pathology Supervisor Penn Medicine Princeton Medical Center Ph: 609.853.6808 Fax: 609.853.6841 -Original Message- From: Laurie Colbert [mailto:boz...@aol.com] Sent: Thursday, May 17, 2018 2:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 Antibody We

Re: [Histonet] p16 Antibody

Hi Laurie, We are purchasing the antibody only from Roche/Ventana and optimizing it for use with our detection system (Bond Refine Detection kit) and the Bond-III stainer. Ask your Ventana person about buying the Ab only. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0

[Histonet] p16 Antibody

We have been using Ventana's p16 antibody and detection kit on an IHC stainer that is not Ventana's stainer.  We have been told that these reagents are now obsolete, but that they can be purchased in dispensers which are customized for Ventana's own stainer.  Can I simply just dispense their

[Histonet] P16 Antibody

We are looking to bring in P16 to do in house but are having a hard time getting the stain to be clean. We are having issues with positive staining on tissue that should be negative. We are working with a concentrated antibody that we have diluted out to 1:6000 (which is 6 times the amount

[Histonet] P16

Does anyone order P16 from Biogenix? I am trying to purchase this antibody from them but can not get ahold of the company to do so. If you do can you please send me the link or phone number to contact them. Thanks -- Charles Riley HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs

Re: [Histonet] p16 from Ventana

Does anybody have more information on what these changes are for the Roche p16? Sent from my iPhone > On Apr 5, 2017, at 16:07, Lynch, Kenia via Histonet > wrote: > > Hi all, > > Roche will still have the p16 antibody, but there are a few changes taking >

Re: [Histonet] p16 from Ventana

Hi all, Roche will still have the p16 antibody, but there are a few changes taking place. Please reach out to your account manager for clarification. Thanks, KCL On Wed, Apr 5, 2017 at 3:47 PM, Teri Johnson via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Wait...what? When is Ventana

Re: [Histonet] p16 from Ventana

Wait...what? When is Ventana p16 being discontinued? Teri Johnson, HT(ASCP)QIHC Manager, Clinical Trial Testing T +1 760 516 5954 tejohn...@genoptix.com Navigate BioPharma, Inc. A Novartis Company 1890 Rutherford Rd. Carlsbad, CA 92008 USA Date: Wed, 5 Apr 2017 01:54:06 + From: Samantha

[Histonet] p16

Good Morning Histonetters, I am wondering how all of you using p16 from Roche on a Leica staining system are going to adapt to the changes coming from Roche. Are you going to pay the exorbitant price for the new kit and list the test as an LDT in your reports, or are you going to source one of

[Histonet] p16 replacements?

Hi Histonet Folks, our current vendor will be discontinuing carrying the antibody for p16. Does anybody have suggestions for replacing in it? We currently use it to stain for CIN. Many thanks in advance for sharing. Cassandra Davis Histology Technician AP Laboratory 302-575-8095

Re: [Histonet] P16

5-2204 Fax From: Dessoye, Michael via Histonet [histonet@lists.utsouthwestern.edu] Sent: Wednesday, June 08, 2016 1:14 PM To: Paula; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] P16 Ventana/Roche bought out MTM which had the patent, so they're the only vendo

Re: [Histonet] P16

Ventana medical systems is the only one I know of right now. On Wed, Jun 8, 2016, 10:47 AM Baldwin, Kathy via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Just saw someone reaching out for P16 was wondering if anyone would share > their protocol for this antibody we are using

[Histonet] P16

Just saw someone reaching out for P16 was wondering if anyone would share their protocol for this antibody we are using Ventana/Roche and are stain is coming out weak :( Thanks S. Kathy Baldwin Histology/Cytology Supervisor Memorial Hospital and Health Care Center 800 West 9th St. Jasper,

Re: [Histonet] P16

To: histonet@lists.utsouthwestern.edu Subject: [Histonet] P16 Hello, Our pathologist would like us to get the P16 antibody. We have the Bond III and we use ready to use antibodies. Leica doesn't carry this antibody and so I was hoping someone can tell me which vendor carries it and that I can order

Re: [Histonet] P16

: [Histonet] P16 Hello, Our pathologist would like us to get the P16 antibody. We have the Bond III and we use ready to use antibodies. Leica doesn't carry this antibody and so I was hoping someone can tell me which vendor carries it and that I can order it from. Thanks in advance for your help

[Histonet] P16

Hello, Our pathologist would like us to get the P16 antibody. We have the Bond III and we use ready to use antibodies. Leica doesn't carry this antibody and so I was hoping someone can tell me which vendor carries it and that I can order it from. Thanks in advance for your help Paula Lab

[Histonet] p16 Nordi QC

There is a fantastic resource for antibody assessment and optimization and they have done p16 as well as many many other antibodies. Their results of testing is free on line. p16 is http://www.nordiqc.org/Epitopes/p16/p16.htm from 2009 with many different companies antibodies. Here is the

[Histonet] P16

Has anyone found a way to do p16 staining without purchasing anything from Ventana? My company wants to do P16 but refuses to by any ventana products and I have explained it is a waste of money to keep testing the antibodies from all the other companies. -- Charles Riley HT(ASCP)CM

[Histonet] P16 alternative

Does anyone know an alternative to Ventana's P16 antibody for testing for viral inclusions like HPV using IHC? -- Charles Riley HT(ASCP)CM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] P16

Hello Histonetters, Happy Friday! I need p16 antibody but not sure which vendor provide reliable antibody. I'd appreciate any recommendation. I also need melanoma and prostate ca control blocks, I can exchange with the once I have. Thank you and have a nice weekend. Kadhem Kadhem

RE: [Histonet] p16

Thanks! From: Shannon Kleiner [mailto:shk...@bellin.org] Sent: Wednesday, March 04, 2015 4:12 PM To: histonet@lists.utsouthwestern.edu; Piche, Jessica Subject: Re: [Histonet] p16 Hello Jessica, We use Cintec p16 on our Bond Max. Here is a link you may find useful http://www.ventana.com

[Histonet] p16

Hi Everyone, I was wondering if people are using the p16 antibody and if so where are you getting it from? Thank you, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This

Re: [Histonet] p16

Ventana - we buy the large test dispenser. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 3/4/15, 12:38 PM, Piche, Jessica jpi...@wtbyhosp.org wrote: Hi Everyone, I was wondering if people are using

Re: [Histonet] p16

Hello Jessica, We use Cintec p16 on our Bond Max. Here is a link you may find useful http://www.ventana.com/cervical Shannon Logan HTL (ASCP) Green Bay, WI Piche, Jessica jpi...@wtbyhosp.org 3/4/2015 1:38 PM Hi Everyone, I was wondering if people are using the p16 antibody and

RE: [Histonet] p16 Antibody

: RE: [Histonet] p16 Antibody We use one from BD, Veronique, with good results. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun

RE: [Histonet] p16 Antibody

[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Véronique Barrès Sent: Wednesday, January 14, 2015 8:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 Antibody Hi Histonetters! I am working in a research lab and we sometimes need to do p16 staining, but our

RE: [Histonet] p16 Antibody

...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sarah.dys...@stdavids.com Sent: Wednesday, January 14, 2015 10:01 AM To: lseb...@uwhealth.org; veronique.bar...@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] p16 Antibody Ventana

RE: [Histonet] p16 Antibody

: RE: [Histonet] p16 Antibody We use the Ventana (INK4a) clone as well. What dilution do you use? Brandy Burnett Histotechnoligist, QIHC(ASCP) CCH Pathology/Histology 508-862-5267 bburn...@capecodhealth.org Expert physicians. Quality hospitals. Superior care. -Original Message

[Histonet] p16 Antibody

Hi Histonetters! I am working in a research lab and we sometimes need to do p16 staining, but our pathologist told us that the antibody we are using right now is not good. I'd like to know which antibody you're using in your labs? Thanks for your help and happy Wednesday! Véronique

[Histonet] p16 [INK4] clone G175-405

Hi Histonetters, I am trying to work up the Biogenex p16 on FFPE tissue and I am not having much luck. Does anyone have any experience with this antibody and getting it to work. I have tried a wide range of dilutions and epitope retrieval (both heat and enzyme). Any suggestions? Thanks in

[Histonet] p16 from XT to Ultra

Hi histoland, You guys were wonderful helping me with the MUM-1 on the XT a few months ago, I am hoping you can help me now. I am trying to optimize the p16 on the Ultra and having a little trouble. It is B-E-A-utiful on the XT but does not want to play nice on the Ultra. Any

[Histonet] p16 from XT to Ultra

-- Message: 3 Date: Thu, 28 Aug 2014 12:37:46 -0400 From: Davis, Cassie cda...@che-east.org Subject: [Histonet] p16 from XT to Ultra To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: be7af02009c3a74f9b8344e70f3655cb0ab5ce3...@chexcms01

[Histonet] p16

We use p16 on the BondMax at a 1:4 dilution. There are 11.5 ml in the container when received. Comes from Dako. Nancy Schmitt HT, MLT(ASCP) Histology Coordinator Dubuque, IA * Message: 12 Date: Thu, 7 Aug 2014 13:06:37 +

RE: [Histonet] p16

...@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Thursday, August 07, 2014 1:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 We use p16 on the BondMax at a 1:4 dilution. There are 11.5 ml in the container when received. Comes from Dako. Nancy Schmitt HT, MLT(ASCP) Histology

[Histonet] P16

Actually comes from Roche..sorry about that. Nancy Schmitt MLT, HT(ASCP) Histology Coordinator NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended

[Histonet] p16

Does anyone know of a comparable clone to the Ventana p16 clone E6H4? I would prefer it to be undiluted (concentrate) so that we can titrate out the antibody ourselves. We need it to have publications that would back this up and/or stained slide pictures I can show our pathologist. Thanks

Re: [Histonet] p16

I have had excellent results with P16 (JC8) from Santa Cruz Biotech. http://www.scbt.com/datasheet-56330-p16-jc8-antibody.html Tony Auge HTL (ASCP) QIHC Histology Supervisor - Chandler Pathology Services Cell: (651) 373-4768 Email: tony.a...@gmail.com

[Histonet] p16

Good Morning! Where are you currently obtaining p16 from? Thanks a bunch! Nancy Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories Please visit our website: http://www.uclaccess.com/ NOTICE: This email may contain legally privileged information. The information

[Histonet] P16

Hi All, I am looking P16 for HP V in Sq.ca of oral cavity, which company and clone will be good. I use Ab cam pathologist not comfortable. Thanks, Muhammad Tahseen Supervisor Histology SKMCHRC Lahore Pakistan ___ Histonet mailing list

[Histonet] p16

Dear Muhammad, We are using MTM p16. This is now supplied by Roche cat# 06594441001. It is only sold as a pre-dilute but I dilute 1/2 and it still works very well. Regards Gary Gracie Senior Technical Officer IHC Laboratory Anatomical Pathology St Vincents Hospital Sydney Australia Hi All, I

[Histonet] p16 control tissue

We are going to be running the p16 antibody from Biocare (RUO) on skin specimens. What type of tissue should I run for a positive control? The data sheet says normal testis.. Laurie Colbert ___ Histonet mailing list

[Histonet] p16 on the Bond

I am having trouble posting to the histonet for some reason. I am looking for condition and incubation times for p16 on the Bond. Our new pathologist says our p16 looks overstained to him and not at all what he is accustomed to seeing and I am trying to get the staining more to his liking,

Re: [Histonet] p16 on the Bond

We dilute the CINtec p16 RTU antibody (Ventana/Roche) 1:10 (15' primary antibody incubation) and use high pH retrieval (H2) for 10' on the Bond Max. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic

Re: [Histonet] P16 on Leica Bond

Really 1:4?___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] P16 on Leica Bond

If anyone out there is performing  p16 on the Leica Bond immunostainer, could you please tell me where you purchase your antibody and what protocol you are using? Thanks so much! Kathy Maddox HT{ASCP} Lake Charles, Louisiana ___ Histonet mailing list

[Histonet] p16

Happy Wednesday Everyone Has anyone been having consistency problems with their p16 staining since MTM has been taken over by Ventana? One day the procedure works another day it doesn't. Same procedure, no variation in the temperature on pretreatment, run on the Dako 48, with no errors.

FW: [Histonet] p16

-celllab.com -Original Message- From: Ross Benik [mailto:r...@premierlab.com] Sent: Wednesday, February 06, 2013 1:26 PM To: Cynthia Pyse Subject: RE: [Histonet] p16 Do you monitor the temperature and humidity inside the Link48? How often do you prepare your retrieval solution/are you using

Re: [Histonet] P16 on Leica Bond

When we obtained the p16 antibody from mtm Laboratories we used it a dilution of 1:10 on the Bond Max. I hope that the concentration will not change now that Ventana is selling it. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Assistant Director, Anatomic Pathology

[Histonet] P16 and HPV

I've been told that the HPV testing will be discontinued as of end of year. HPV expresses the P16 gene but so does other type of cancers associated with HPV. It is possible to have a P16 pos and HPV negative test. But is it not possible to have HPV pos and P16 neg. if HPV is positive then the

[Histonet] p16

P16 stain demonstrates week nuclear stain. Any ideas how to correct? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] p16

In order to troubleshoot, one would need to know the following: Test tissue Fixation Antibody clone, dilution, and incubation time Detection system Antigen retrieval Positive control tissue Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection

RE: [Histonet] p16

, July 20, 2012 12:17 PM To: Gerald Davydov; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] p16 In order to troubleshoot, one would need to know the following: Test tissue Fixation Antibody clone, dilution, and incubation time Detection system Antigen retrieval Positive control tissue

RE: [Histonet] p16

-boun...@lists.utsouthwestern.edu] On Behalf Of Britton, Josette C [jcbrit...@cheshire-med.com] Sent: Friday, July 20, 2012 12:57 PM To: Richard Cartun; Gerald Davydov; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] p16 Where is everyone getting their p16 IVD antibody from? Josie

RE: [Histonet] p16

The only IVD p16 available in the US is from MTM. However, Roche just bought MTM so you will have to order it through Ventana. Their Customer support number is 800-227-2155. Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical

[Histonet] P16

What are folks using for P16? Thanks for your help- Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have

[Histonet] p16 Protocol....

Hello all, I was wondering if anyone would be willing to share a protocol for p16 (Biogenix pre-dilute) antibody on the Benchmark XT. I'm having a little trouble getting anything to stain. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163

[Histonet] P16 and ProExC Substitutions

Hi All, Now that MTM has made P16 very expensive and TriPath is have problems producing ProExC, what's everybody using on ffpe tissue? We were told by TriPath/BD that ProExC will probably not be available until late June. Rick Boen, BS, HTL(ASCP) St. Luke's Hospital 901 East 1st

[Histonet] p16 protocol for VMS instruments

For another method that works on Ventana XTs and BenchMarks: BD antibody (cat.#: 550834), 1:10, mild CC1, 32 incubation @ 42 degrees, Hem II/8 Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596

[Histonet] p16 antibody

Hi everyone was wondering who carries the IVD p16 antibody? Is it only MTM? Thanks in advance. Michele Carr HTL ASCP Medical Laboratory Services ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] p16 antibody

Yes. They hold the patent. On Feb 10, 2011 1:30 PM, Michele Carr michelecar...@yahoo.com wrote: Hi everyone was wondering who carries the IVD p16 antibody? Is it only MTM? Thanks in advance. Michele Carr HTL ASCP Medical Laboratory Services ___

RE: [Histonet] p16 antibody

From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michele Carr [michelecar...@yahoo.com] Sent: Thursday, February 10, 2011 1:29 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 antibody Hi

Re: [Histonet] p16 antibody

They hold the patent and no one else produces it. We order the kit and use the antibody on the Ventana XT stainers, placing the antibody in a prep kit. Works great. Mark Turner, PhD, HT(ASCP)QIHC Mayo Clinic in Arizona Michele Carr michelecar...@yahoo.com wrote: Hi everyone was

[Histonet] p16 antibody

Hello all I am relatively new to IHC and my pathologist is requesting the p16 antibody, I have not been able to find it at any of the vendors we work with. I did a google search and saw that MTM labs has it but it seems as though they sell it as a kit. Does anyone know where I could find this

RE: [Histonet] p16 antibody

@lists.utsouthwestern.edu Subject: [Histonet] p16 antibody Hello all I am relatively new to IHC and my pathologist is requesting the p16 antibody, I have not been able to find it at any of the vendors we work with. I did a google search and saw that MTM labs has it but it seems as though they sell it as a kit. Does

RE: [Histonet] p16 antibody

: Thursday, October 07, 2010 1:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 antibody Hello all I am relatively new to IHC and my pathologist is requesting the p16 antibody, I have not been able to find it at any of the vendors we work with. I did a google search and saw

Re: [Histonet] p16

Hi Deanne, My understanding is that you must get p16 from MTM Laboratories. They have some sort of monopoly right now. I still have some leftover from Cell Marque and when I run low or the antibody is about to expire I must also go to MTM. I currently use the Cell Marque p16 Ready To Use by

RE: [Histonet] p16

Of Knutson, Deanne Sent: Tuesday, March 09, 2010 5:28 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] p16 Hello fellow Histonetters, Does anyone run the antibody p16? If so, where do you purchase it from and maybe you can share your protocol with me? Thank you in advance. Deanne

[Histonet] p16

Hello fellow Histonetters, Does anyone run the antibody p16? If so, where do you purchase it from and maybe you can share your protocol with me? Thank you in advance. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center (701)-530-6730

[Histonet] p16

Anyone know where I can get an IVD p16? Roxanne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet