I am trying to find the most appropriate pan B-cell marker for IHC on
frozen mouse tissue. I know that CD45R/B220 used to be the most commonly
used, but recent publications suggest PAX5 or CD19 as more restricted to
the B-cell lineage. Does anyone have any other suggestions, or are these
generally
B220 is a pretty good B-cell marker, although you're right that it's not
restricted to the B-cell lineage. It's also expressed in some T-cells and NK
cells, for example. Cells that are double positive for B220 and IgG or IgM
are B-cells almost exclusively. CD19 is a good marker for mature B-cells,
