After macro trimming into our blocks (very gently if friable or dry) we
place them on an ice tray to soak for a good while.
Each well is filled with a little bit of water - this rehydrates the
tissue just enough to get a few good sections off the top.
we¹ve had success with this method for most
This is common with mouse and rat tissues, they get over-dried with a
typical processing schedule.
Soaking the face of the block with a kimwipe wet with ice water for 60
-120 seconds will enable you to cut 10 nice sections, maybe more.
Geoff
On 2/5/2015 6:23 AM, Emily Brown wrote:
Hello all!
Yes, exactly what Mike and Geoff said. All mouse tissue, especially liver, can
be really dry and needs a 'soak'. I have left them for an hour before now but
don't leave it for longer than 4 hours though because it can start to swell and
de-process!
You will still only get a few non-chattery
: Re: [Histonet] paraffin sectioning-dry tissue?
Yes, exactly what Mike and Geoff said. All mouse tissue, especially liver, can
be really dry and needs a 'soak'. I have left them for an hour before now but
don't leave it for longer than 4 hours though because it can start to swell and
de-process
:
histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Caroline Miller
Sent: Thursday, February 05, 2015 6:41 AM
To: Geoff
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] paraffin sectioning-dry tissue?
Yes, exactly what Mike and Geoff said. All mouse tissue, especially liver
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey
Sent: Thursday, February 05, 2015 10:22 AM
To: James Watson
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] paraffin sectioning-dry tissue?
By adding water, ice, or warm humidity (through exhalations) to the mix,
though
