Re: [Histonet] paraffin sectioning problems

2008-09-30 Thread Rene J Buesa
Mon, 9/29/08, James Dooley <[EMAIL PROTECTED]> wrote: From: James Dooley <[EMAIL PROTECTED]> Subject: [Histonet] paraffin sectioning problems To: histonet@lists.utsouthwestern.edu Date: Monday, September 29, 2008, 7:12 PM I recently started paraffin sectioning and I am having trouble

[Histonet] paraffin sectioning problems

2008-09-29 Thread James Dooley
I recently started paraffin sectioning and I am having trouble with getting good quality sections reproducibly. I was wondering if you have any tips that may help. I am sectioning thymus, pancreas, and lung. Section thickness is 10 um. The microtome that I am using is Reichert-Jung Supercut, dis