Ashley,
Phosphatases are relatively resistant to fixation which is why the
histochemical phosphatase methods work. You may want to try putting
phosphatase inhibitors in you fix or post-fix solutions to stop
dephosphorylation, and see if it makes a difference in your ihc. I
think Sigma has a
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of MKing
Sent: Monday, April 19, 2010 8:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: SPAM-LOW: [Histonet] phosphoantibodies
Ashley,
Phosphatases are relatively resistant to fixation which is why
