[Histonet] RE: Xylene Substitute Protocols

Statlab XS-3 & Acrymount. I do H & E staining only. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hilliard, Dawud Deshawn Sent: Thursday, July 24, 2014 9:50 AM To: histonet@lists.utsouthwestern.edu Subj

[Histonet] RE: Xylene Substitute Protocols

Richard Allen mounting media REF 4111. >>> Shannon Kleiner 7/24/2014 12:59 PM >>> Formula 83 in first three stations and last four stations on the Leica XL stainer. >>> "Wineman, Terra" 7/24/2014 12:06 PM >>> We use Richard Allan Clear-Rite 5min * 3 stations with any xylene based mountin

[Histonet] RE: Xylene Substitute Protocols

Formula 83 in first three stations and last four stations on the Leica XL stainer. >>> "Wineman, Terra" 7/24/2014 12:06 PM >>> We use Richard Allan Clear-Rite 5min * 3 stations with any xylene based mounting type Terra Wineman, HTL(ASCP)cm Novus International -Original Message- Fro

[Histonet] RE: Xylene Substitute Protocols

We use Richard Allan Clear-Rite 5min * 3 stations with any xylene based mounting type Terra Wineman, HTL(ASCP)cm Novus International -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hilliard, Dawud Deshaw

[Histonet] RE: Xylene substitute for processing GI biopsies

Joe, Yes. We are about to go xylene-free (though we are still planning on using if for cleaning, because the feedback we got was that we still needed to clean the processors with xylene or we would run into problems, and my supervisor heard from people that they had to go back to xylene flushing

[Histonet] RE: Xylene testing ANP 08216

b.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Tuesday, April 15, 2014 11:41 AM To: Piche, Jessica; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Xylene testing ANP 08216 We

[Histonet] RE: Xylene testing ANP 08216

du Subject: [Histonet] RE: Xylene testing ANP 08216 We test every six months. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.han...@parrishmed.com ---

[Histonet] RE: Xylene testing ANP 08216

We test every six months. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.han...@parrishmed.com -Original Message- From: histonet-boun...@lis

[Histonet] RE: Xylene testing ANP 08216

We test every year. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premi

[Histonet] RE: Xylene-free (isopropanol) processing protocol

What type of processing instrument are you using? Donna Willis, HT/HTL (ASCP) Anatomic Pathology Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.wil...@baylorhealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.e

Re: [Histonet] RE: Xylene Substitutes

I like ProPar from Anatech.Margie Chadwell Sent from my Verizon Wireless 4G LTE DROID "Grantham Andrea L - (algranth)" wrote: I'm using Clear Rite 3 and I like it. Is there a better xylene substitute for animal tissues? Andrea Grantham, HT (ASCP) Senior Research Specialist University of A

[Histonet] RE: Xylene Substitutes

We prefer Formula 83 from CBG Biotech Thanks, Liz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] RE: Xylene Substitutes

-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Wednesday, August 21, 2013 8:59 AM To: Grantham, Andrea L - (algranth) Cc: HISTONET Subject: RE: [Histonet] RE: Xylene Substitutes We use Propar, mostly for mouse and rat eye samples, it works we

[Histonet] RE: Xylene Substitutes

I use XS-3 from Statlab for my H&E staining & Iso Alc for my overnight derm processing. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yaskovich, Ruth A (NIH/NIDCR) [E] Sent: Wednesday, August 21, 2013 8

RE: [Histonet] RE: Xylene Substitutes

AM Cc: HISTONET Subject: Re: [Histonet] RE: Xylene Substitutes I'm using Clear Rite 3 and I like it. Is there a better xylene substitute for animal tissues? Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Labor

Re: [Histonet] RE: Xylene Substitutes

I'm using Clear Rite 3 and I like it. Is there a better xylene substitute for animal tissues? Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu

[Histonet] RE: Xylene Substitutes

Just my 2 cents: we've tried several and like CitriSolv from Fisher. Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fa

RE: [Histonet] RE: Xylene/Paint Thinner

#x27;White, Lisa M.'; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] RE: Xylene/Paint Thinner If you ever need to degrease any auto part, just place it in water, add liquid soap to about 5% conc., and heat it until boiling and let them in boiling water during 5 minutes. Wa

Re: [Histonet] RE: Xylene/Paint Thinner

To: "'White, Lisa M.'" ; "'histonet@lists.utsouthwestern.edu'" Sent: Thursday, February 7, 2013 11:06 AM Subject: [Histonet] RE: Xylene/Paint Thinner I don't know if the auto parts stores carry xylene, but it is an awesome degreaser. Learned that fro

RE: [Histonet] RE: Xylene/Paint Thinner

8:14 AM To: Victor A. Tobias; 'White, Lisa M.'; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] RE: Xylene/Paint Thinner If you ever need to degrease any auto part, just place it in water, add liquid soap to about 5% conc., and heat it until boiling and let them in

[Histonet] RE: Xylene/Paint Thinner

I don't know if the auto parts stores carry xylene, but it is an awesome degreaser. Learned that from my mentor back in the 70's. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtob...@u.washington.edu 206-744

[Histonet] Re: Xylene Substitutes

Adrienne Anderson (where?) asks: >>My lab is looking into xylene substitutes, and I'd love some feedback on what >>other labs are using. We currently use SubX, but are there other items out >>there more economical?<< I never heard of SubX, but the Leica Microsystems Richmond [no kin!] Inc. MSDS

Re: [Histonet] RE: xylene substitute for processing

2 07:58 AM To "'Masood, Sameena Kathryn'" , "Cross, Kelly" , "Histonet Listserv (E-mail) (histonet@lists.utsouthwestern.edu)" cc Subject [Histonet] RE: xylene substitute for processing Some people have issues with the smell and the other option is Ana

Re: [Histonet] RE: xylene substitute for processing

I have been using Clear Rite 3 for years on animal tissue in a VIP for overnite processing and it is great. I get it from Fisher. There has never been a problem with special stains but I use xylene on my stain lines. Andi Grantham On Mar 7, 2012, at 8:35 AM, Masood, Sameena Kathryn wrote: >

[Histonet] RE: xylene substitute for processing

estern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Masood, Sameena Kathryn Sent: Wednesday, March 07, 2012 9:36 AM To: Cross, Kelly; Histonet Listserv (E-mail) (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: xylene substitute for processing Kelly, Histo-Clear can b

[Histonet] RE: xylene substitute for processing

Kelly, Histo-Clear can be directly substituted for Xylene at every point in processing and staining. It's completely non-toxic (even has a food grade rating), is made from oranges, and smells delicious. You don't have to work in a hood and it seems to make tissue less brittle than Xylene also.

[Histonet] RE: xylene substitute for processing

I use the Shandon Excelsior and use an overnight run with Iso Alcohol and only use Xylene in the cleaning cycle. Our Thermo Rep came out and set it all up for us. No problems with the processing. I also use sub xylene in staining with a few extra steps but no problems. -Original Message---

[Histonet] re: xylene substitute for processing

We have been processing without xylene on ThermoFisher Excelsior processors for a couple of years. We go directly from 100% isopropyl alcohol into paraffin. A thermo sales rep should be able to give you some literature on this. When we first got the processors we did side by side validation

[Histonet] Re: xylene and preggers

Sarah, To answer your first question, what is .75%? The permissible exposure limit for xylene is 100 ppm (435 mg/cubic meter of air) for an 8-hour time-weighted average, and 150 ppm (655 mg/m3) for a 15 minute short term exposure limit. How does .75% fit in to that? (Sorry to answer a question

[Histonet] RE: Xylene sensitivity

Hi Tom, Yes, our Employee Health & Safety department is involved with this problem. Our lab is negative pressure, but do not know the air exchange rate. I will definitely ask that to be checked. We monitor employees annually and had a full environmental assessment 2-3 years ago prior to ins

[Histonet] RE: xylene sensitivity

Kathryn, Thanks for the advice. I will evaluate the installation of a hood. Regards, Shelley D'Attilio MT(ASCP) Manager, Chemistry, Cytology and Histology Dept. of Pathology and Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas -Original Message- From: Stoll, Kathryn [ma

[Histonet] Re: Xylene sensitivity

Some points that haven't been mentioned in this discussion: I think most labs have switched from xylenes to aliphatic hydrocarbons for processing, hydration, and dehydration though obviously considerable controversy remains. (I can't imagine anybody switching from xylene to limonene now - particul

[Histonet] Re: Xylene re-use SOP

Just to answer a few more questions people asked. We do not use a solvent recycler. Once our xylene is "bad," we label it with a sticker and EH&S picks it up and disposes of it somehow. The graduate student who started this would just eyeball the xylene and if it was gunky, replace it with fresh x

Re: [Histonet] Re: xylene substitute

xylene substitutes also require more time than xylene "Johnson, Teri" Sent by: histonet-boun...@lists.utsouthwestern.edu 10/27/2010 10:13 AM To "'histonet@lists.utsouthwestern.edu'" cc Subject [Histonet] Re: xylene substitute Hi all, I just wanted

[Histonet] RE: xylene substitute

We use a product called pro-par, produced by Anatech. We don't use permount routinely, but a few of our specials require it. We dehydrate and then clear with the pro-par, and it works fine for those stains. It is much less harsh on tissue than xylene; we have three VIP's, and our specimens sit i

[Histonet] Re: Xylene Substitute

Sheila Hass at Micro Path Laboratories [where?} asks: >>Could someone recommend a xylene substitute and mounting media to use for staining and coverslipping (no vendors please)? I am considering processing without xylene, and would like to discontinue it everywhere if possible.<< We've discussed t

RE: [Histonet] Re: Xylene substitutes (UNCLASSIFIED)

To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Xylene substitutes William (Bill) O'Donnell, HT (ASCP) QIHC, Lead Histologist, Good Samaritan Hospital, Kearney, Nebraska, notes that Clear-Rite 3 is at least temporarily unavailable, and he is looking for a substitute. (Anybody

[Histonet] Re: Xylene substitutes

William (Bill) O'Donnell, HT (ASCP) QIHC, Lead Histologist, Good Samaritan Hospital, Kearney, Nebraska, notes that Clear-Rite 3 is at least temporarily unavailable, and he is looking for a substitute. (Anybody know what's going on with Clear-Rite 3?) Clear-Rite 3 is an aliphatic (rather than a lim

[Histonet] re: Xylene

I don't know whether you are decalcifying tissue or not, but I have had similar problems with tissue that was over-decalcified. It was particularly bad when decalcified with commercial "decalcifying agent" which contained formic along with several chelating agents. The end result was tissue that

[Histonet] Re: Xylene substitute

You can view the MSDS for CBG Biotech's "Formula 83" xylene substitute at www.cbgbiotech.com/msds/MSDS-Formula83071007.pdf It's described as a "naphthenic hydrocarbon blend". I'd note the low flash point (7 C, 45 F), considerably lower than xylene's. Other aliphatic or naphthenic (cycloalkane) m

[Histonet] RE: Xylene substitute for automated coverslipper

Jim, I use Formula 83 from CBG on a Richard Allan CTM 6 glass coverslipper with no problems. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 293-4424 ext 7118 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.u

Re: [Histonet] Re: Xylene substitute

estern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, January 22, 2009 9:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Xylene substitute Questions about xylene substitutes: This has been discussed a good many times on Histonet.

RE: [Histonet] Re: Xylene substitute

tonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, January 22, 2009 9:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Xylene substitute Questions about xylene substitutes: This has been discussed a good man

[Histonet] Re: Xylene substitute

Questions about xylene substitutes: This has been discussed a good many times on Histonet. There are two classes of xylene substitutes: Limonene and turpentine are terpenes. Several brands; AmeriClear was mentioned. Limonene is prepared by steam distillation of orange peels. It has a strong citru

[Histonet] RE: Xylene substitute

Tom, We are using Formula 83 from CBG and like it a lot. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@l