Statlab XS-3 & Acrymount. I do H & E staining only.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hilliard, Dawud
Deshawn
Sent: Thursday, July 24, 2014 9:50 AM
To: histonet@lists.utsouthwestern.edu
Subj
Richard Allen mounting media REF 4111.
>>> Shannon Kleiner 7/24/2014 12:59 PM >>>
Formula 83 in first three stations and last four stations on the Leica XL
stainer.
>>> "Wineman, Terra" 7/24/2014 12:06 PM >>>
We use Richard Allan Clear-Rite 5min * 3 stations with any xylene based
mountin
Formula 83 in first three stations and last four stations on the Leica XL
stainer.
>>> "Wineman, Terra" 7/24/2014 12:06 PM >>>
We use Richard Allan Clear-Rite 5min * 3 stations with any xylene based
mounting type
Terra Wineman, HTL(ASCP)cm
Novus International
-Original Message-
Fro
We use Richard Allan Clear-Rite 5min * 3 stations with any xylene based
mounting type
Terra Wineman, HTL(ASCP)cm
Novus International
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hilliard, Dawud
Deshaw
Joe,
Yes. We are about to go xylene-free (though we are still planning on using if
for cleaning, because the feedback we got was that we still needed to clean the
processors with xylene or we would run into problems, and my supervisor heard
from people that they had to go back to xylene flushing
b.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth
Chlipala
Sent: Tuesday, April 15, 2014 11:41 AM
To: Piche, Jessica; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Xylene testing ANP 08216
We
du
Subject: [Histonet] RE: Xylene testing ANP 08216
We test every six months.
Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.han...@parrishmed.com
---
We test every six months.
Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.han...@parrishmed.com
-Original Message-
From: histonet-boun...@lis
We test every year.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com
March 10, 2014 is Histotechnology Professionals Day
Ship to Address:
Premi
What type of processing instrument are you using?
Donna Willis, HT/HTL (ASCP)
Anatomic Pathology Manager
Baylor University Medical Center-Dallas
ph. 214-820-2465 office
ph. 214-725-6184 mobile
donna.wil...@baylorhealth.edu
-Original Message-
From: histonet-boun...@lists.utsouthwestern.e
I like ProPar from Anatech.Margie Chadwell
Sent from my Verizon Wireless 4G LTE DROID
"Grantham Andrea L - (algranth)" wrote:
I'm using Clear Rite 3 and I like it. Is there a better xylene substitute for
animal tissues?
Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of A
We prefer Formula 83 from CBG Biotech
Thanks,
Liz
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-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth
Chlipala
Sent: Wednesday, August 21, 2013 8:59 AM
To: Grantham, Andrea L - (algranth)
Cc: HISTONET
Subject: RE: [Histonet] RE: Xylene Substitutes
We use Propar, mostly for mouse and rat eye samples, it works we
I use XS-3 from Statlab for my H&E staining & Iso Alc for my overnight derm
processing.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yaskovich, Ruth
A (NIH/NIDCR) [E]
Sent: Wednesday, August 21, 2013 8
AM
Cc: HISTONET
Subject: Re: [Histonet] RE: Xylene Substitutes
I'm using Clear Rite 3 and I like it. Is there a better xylene substitute for
animal tissues?
Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Labor
I'm using Clear Rite 3 and I like it. Is there a better xylene substitute for
animal tissues?
Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724
algra...@email.arizona.edu
Just my 2 cents: we've tried several and like CitriSolv from Fisher.
Peggy Sherwood
Research Specialist, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fa
#x27;White, Lisa M.'; 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] RE: Xylene/Paint Thinner
If you ever need to degrease any auto part, just place it in water, add liquid
soap to about 5% conc., and heat it until boiling and let them in boiling water
during 5 minutes. Wa
To: "'White, Lisa M.'" ;
"'histonet@lists.utsouthwestern.edu'"
Sent: Thursday, February 7, 2013 11:06 AM
Subject: [Histonet] RE: Xylene/Paint Thinner
I don't know if the auto parts stores carry xylene, but it is an awesome
degreaser. Learned that fro
8:14 AM
To: Victor A. Tobias; 'White, Lisa M.'; 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] RE: Xylene/Paint Thinner
If you ever need to degrease any auto part, just place it in water, add liquid
soap to about 5% conc., and heat it until boiling and let them in
I don't know if the auto parts stores carry xylene, but it is an awesome
degreaser. Learned that from my mentor back in the 70's.
Victor
Victor Tobias HT(ASCP)
Clinical Applications Analyst
Harborview Medical Center
Dept of Pathology Room NJB244
Seattle, WA 98104
vtob...@u.washington.edu
206-744
Adrienne Anderson (where?) asks:
>>My lab is looking into xylene substitutes, and I'd love some feedback on what
>>other labs are using. We currently use SubX, but are there other items out
>>there more economical?<<
I never heard of SubX, but the Leica Microsystems Richmond [no kin!]
Inc. MSDS
2 07:58 AM
To
"'Masood, Sameena Kathryn'" , "Cross,
Kelly" , "Histonet Listserv (E-mail)
(histonet@lists.utsouthwestern.edu)"
cc
Subject
[Histonet] RE: xylene substitute for processing
Some people have issues with the smell and the other option is Ana
I have been using Clear Rite 3 for years on animal tissue in a VIP for overnite
processing and it is great. I get it from Fisher. There has never been a
problem with special stains but I use xylene on my stain lines.
Andi Grantham
On Mar 7, 2012, at 8:35 AM, Masood, Sameena Kathryn wrote:
>
estern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Masood, Sameena
Kathryn
Sent: Wednesday, March 07, 2012 9:36 AM
To: Cross, Kelly; Histonet Listserv (E-mail) (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] RE: xylene substitute for processing
Kelly,
Histo-Clear can b
Kelly,
Histo-Clear can be directly substituted for Xylene at every point in processing
and staining. It's completely non-toxic (even has a food grade rating), is
made from oranges, and smells delicious. You don't have to work in a hood and
it seems to make tissue less brittle than Xylene also.
I use the Shandon Excelsior and use an overnight run with Iso Alcohol and only
use Xylene in the cleaning cycle. Our Thermo Rep came out and set it all up for
us. No problems with the processing. I also use sub xylene in staining with a
few extra steps but no problems.
-Original Message---
We have been processing without xylene on ThermoFisher Excelsior processors for
a couple of years. We go directly from 100% isopropyl alcohol into paraffin.
A thermo sales rep should be able to give you some literature on this.
When we first got the processors we did side by side validation
Sarah,
To answer your first question, what is .75%? The permissible exposure limit for
xylene is 100 ppm (435 mg/cubic meter of air) for an 8-hour time-weighted
average, and 150 ppm (655 mg/m3) for a 15 minute short term exposure limit. How
does .75% fit in to that? (Sorry to answer a question
Hi Tom,
Yes, our Employee Health & Safety department is involved with this problem.
Our lab is negative pressure, but do not know the air exchange rate. I will
definitely ask that to be checked.
We monitor employees annually and had a full environmental assessment 2-3 years
ago prior to ins
Kathryn,
Thanks for the advice. I will evaluate the installation of a hood.
Regards,
Shelley D'Attilio MT(ASCP)
Manager, Chemistry, Cytology and Histology
Dept. of Pathology and Laboratory Medicine
Stormont-Vail HealthCare
Topeka, Kansas
-Original Message-
From: Stoll, Kathryn [ma
Some points that haven't been mentioned in this discussion:
I think most labs have switched from xylenes to aliphatic hydrocarbons
for processing, hydration, and dehydration though obviously
considerable controversy remains. (I can't imagine anybody switching
from xylene to limonene now - particul
Just to answer a few more questions people asked. We do not use a solvent
recycler. Once our xylene is "bad," we label it with a sticker and EH&S
picks it up and disposes of it somehow.
The graduate student who started this would just eyeball the xylene and if
it was gunky, replace it with fresh x
xylene substitutes also require more time than xylene
"Johnson, Teri"
Sent by: histonet-boun...@lists.utsouthwestern.edu
10/27/2010 10:13 AM
To
"'histonet@lists.utsouthwestern.edu'"
cc
Subject
[Histonet] Re: xylene substitute
Hi all,
I just wanted
We use a product called pro-par, produced by Anatech. We don't use permount
routinely, but a few of our specials require it. We dehydrate and then
clear with the pro-par, and it works fine for those stains. It is much less
harsh on tissue than xylene; we have three VIP's, and our specimens sit i
Sheila Hass at Micro Path Laboratories [where?} asks: >>Could someone
recommend a xylene substitute and mounting media to use for staining
and coverslipping (no vendors please)? I am considering processing
without xylene, and would like to discontinue it everywhere if
possible.<<
We've discussed t
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Xylene substitutes
William (Bill) O'Donnell, HT (ASCP) QIHC, Lead Histologist, Good
Samaritan Hospital, Kearney, Nebraska, notes that Clear-Rite 3 is at
least temporarily unavailable, and he is looking for a substitute.
(Anybody
William (Bill) O'Donnell, HT (ASCP) QIHC, Lead Histologist, Good
Samaritan Hospital, Kearney, Nebraska, notes that Clear-Rite 3 is at
least temporarily unavailable, and he is looking for a substitute.
(Anybody know what's going on with Clear-Rite 3?)
Clear-Rite 3 is an aliphatic (rather than a lim
I don't know whether you are decalcifying tissue or not, but I have had
similar problems with tissue that was over-decalcified. It was particularly
bad when decalcified with commercial "decalcifying agent" which contained
formic along with several chelating agents. The end result was tissue that
You can view the MSDS for CBG Biotech's "Formula 83" xylene substitute at
www.cbgbiotech.com/msds/MSDS-Formula83071007.pdf
It's described as a "naphthenic hydrocarbon blend".
I'd note the low flash point (7 C, 45 F), considerably lower than
xylene's. Other aliphatic or naphthenic (cycloalkane) m
Jim,
I use Formula 83 from CBG on a Richard Allan CTM 6 glass coverslipper with no
problems.
Linda
Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Phone: (937) 293-4424 ext 7118
Email: lbla...@digestivespecialists.com
-Original Message-
From: histonet-boun...@lists.u
estern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond
Sent: Thursday, January 22, 2009 9:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Xylene substitute
Questions about xylene substitutes: This has been discussed a good
many times on Histonet.
tonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond
Sent: Thursday, January 22, 2009 9:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Xylene substitute
Questions about xylene substitutes: This has been discussed a good
man
Questions about xylene substitutes: This has been discussed a good
many times on Histonet.
There are two classes of xylene substitutes:
Limonene and turpentine are terpenes. Several brands; AmeriClear was
mentioned. Limonene is prepared by steam distillation of orange peels.
It has a strong citru
Tom,
We are using Formula 83 from CBG and like it a lot.
Linda
Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email: lbla...@digestivespecialists.com
-Original Message-
From: histonet-boun...@l
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