;
Foshey, Annette
Subject: Re: [Histonet] Tissue falling off slides
1) Try tap water
2) Try hydrophilic IHC slides, (I will send you samples for free)
Ken
kdea...@hotmail.com<mailto:kdea...@hotmail.com>
-Original Message-
From: richard cartun via Histonet
mailto:hi
] Tissue falling off slides
Hi Annette!
Do you put anything else in your water bath besides deionized water?
Richard CartunMorphologic Proteomics, LLC(860) 490-7633
On Tuesday, January 30, 2024 at 03:14:50 PM EST, Foshey, Annette via
Histonet wrote:
I work with whole mount prostate blocks
Hi Annette!
Do you put anything else in your water bath besides deionized water?
Richard CartunMorphologic Proteomics, LLC(860) 490-7633
On Tuesday, January 30, 2024 at 03:14:50 PM EST, Foshey, Annette via
Histonet wrote:
I work with whole mount prostate blocks that I cut and stain
I work with whole mount prostate blocks that I cut and stain for IHC manually.
I use superfrost plus slides that I purchase from the Brain Research
Laboratories. I airdry the slides after sectioning and then they are placed in
a rack and dried in a 60 degree oven overnight. On some of the
I forgot to mention that we are using a 3 in one HIER for target retrieval,
instead of running
down (xylene-ETOH). Could this also contribute to tissue falling off?
~~
This email and any files transmitted with it are
Our lab has encountered an issue with tissue falling off of the slides. It is
mostly the patient tissue
that is falling off. Some of the Techs are using control slides that have been
pre-cut and I am wondering
if this might be part of the problem. Any information would be very much
Good morning histonetters!!
I received a project from an outside source to process and embed mouse hearts
for this group. From what I could gather the specimens had varying times in 4%
paraformaldehyde from 24-48 hours all the way up to 4 months. Once processed
and embedded the specimens where
To: histonet@lists.utsouthwestern.edu
Cc: Betsy Woessner; Kathryn Gizzi
Subject: [Histonet] Tissue falling off slides
Good morning histonetters!!
I received a project from an outside source to process and embed mouse
hearts for this group. From what I could gather the specimens had
varying times in 4