Re: [Histonet] Tissue falling off slides

2024-01-31 Thread Rachel Gonzalez via Histonet
; Foshey, Annette Subject: Re: [Histonet] Tissue falling off slides 1) Try tap water 2) Try hydrophilic IHC slides, (I will send you samples for free) Ken kdea...@hotmail.com<mailto:kdea...@hotmail.com> -Original Message- From: richard cartun via Histonet mailto:hi

Re: [Histonet] Tissue falling off slides

2024-01-31 Thread Ken M via Histonet
] Tissue falling off slides Hi Annette! Do you put anything else in your water bath besides deionized water? Richard CartunMorphologic Proteomics, LLC(860) 490-7633 On Tuesday, January 30, 2024 at 03:14:50 PM EST, Foshey, Annette via Histonet wrote: I work with whole mount prostate blocks

Re: [Histonet] Tissue falling off slides

2024-01-31 Thread richard cartun via Histonet
Hi Annette! Do you put anything else in your water bath besides deionized water? Richard CartunMorphologic Proteomics, LLC(860) 490-7633 On Tuesday, January 30, 2024 at 03:14:50 PM EST, Foshey, Annette via Histonet wrote: I work with whole mount prostate blocks that I cut and stain

[Histonet] Tissue falling off slides

2024-01-30 Thread Foshey, Annette via Histonet
I work with whole mount prostate blocks that I cut and stain for IHC manually. I use superfrost plus slides that I purchase from the Brain Research Laboratories. I airdry the slides after sectioning and then they are placed in a rack and dried in a 60 degree oven overnight. On some of the

[Histonet] tissue falling off slides

2014-08-19 Thread Burnett, Brandy
I forgot to mention that we are using a 3 in one HIER for target retrieval, instead of running down (xylene-ETOH). Could this also contribute to tissue falling off? ~~ This email and any files transmitted with it are

[Histonet] tissue falling off slides

2014-08-15 Thread Burnett, Brandy
Our lab has encountered an issue with tissue falling off of the slides. It is mostly the patient tissue that is falling off. Some of the Techs are using control slides that have been pre-cut and I am wondering if this might be part of the problem. Any information would be very much

[Histonet] Tissue falling off slides

2009-11-10 Thread John Shelley
Good morning histonetters!! I received a project from an outside source to process and embed mouse hearts for this group. From what I could gather the specimens had varying times in 4% paraformaldehyde from 24-48 hours all the way up to 4 months. Once processed and embedded the specimens where

RE: [Histonet] Tissue falling off slides

2009-11-10 Thread Weems, Joyce
To: histonet@lists.utsouthwestern.edu Cc: Betsy Woessner; Kathryn Gizzi Subject: [Histonet] Tissue falling off slides Good morning histonetters!! I received a project from an outside source to process and embed mouse hearts for this group. From what I could gather the specimens had varying times in 4