I favor the VIP too. My lab has 4 VIPs and 1 Leica Peloris. The VIPs can
process more cassettes on a run and cost a lot less than the Peloris.
Paula
Sent from my iPhone
> On Mar 6, 2020, at 12:34 AM, warda hassan via Histonet
> wrote:
>
> Hello to all histonets
>
> Advanced thanking
Hello to all histonets
Advanced thanking to whoever will give me a feedback on their selection for
tissue processing
We are to purchase a new tissue processing
List are
Histo pro 200 & 300( histoline)
Milstone
Diapth
Shandon - Thermo Revos
Sakura VIP TEK6
Any feedback on your hand-on experiences
Hello,
Does anyone have any tissue processing baskets for the Sakura-small 50
cassettes basket size that they no longer need and want to get rid of? If so,
I'd be happpy to put them to good use. Thanks.
Respectfully,
Pamela Romundstad HT (ASCP), QIHCCM
Lead Histology Technician
Gundersen
My lab just recently purchased a Tissue-Tek VIP 6 AI processor and I was
wondering if anyone would be willing to share their tissue processing
protocols. We process small G i biopsies as well as large skins and breast
specimens. Trying to get a good idea about where to start my processing
testing.
: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue processing question
Hello all,
I was wondering what everyone uses to secure biopsy and scant tissues through
processing. Also what would you recommend placing breast cores in for
processing. Having an argument with grossing staff and pathologist abou
Hello all,
I was wondering what everyone uses to secure biopsy and scant tissues
through processing. Also what would you recommend placing breast cores in
for processing. Having an argument with grossing staff and pathologist
about whether to use sponges, tissue paper, or something else. Looking
.
From: Caroline Miller [mailto:mi...@3scan.com]
Sent: Friday, January 29, 2016 1:36 PM
To: Walter Benton <wben...@cua.md>
Cc: Charles Riley <cri...@dpspa.com>; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Tissue processing question
I really like this type:
https://www.f
althcare
> Magazines.
>
>
>
> -Original Message-
> From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu
> ]
> Sent: Friday, January 29, 2016 12:43 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Tissue processing questio
, 2016 1:36 PM
To: Walter Benton <wben...@cua.md>
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Tissue processing question
I really like this type:
https://www.fishersci.com/shop/products/starplex-scientific-histoplex-tissue-cassettes-micromesh-chamber-8/p-2782584
(although I bu
;Ultimately, get samples of whatever you like to use.
>
>From: Caroline Miller [mailto:mi...@3scan.com]
>Sent: Friday, January 29, 2016 1:36 PM
>To: Walter Benton <wben...@cua.md>
>Cc: Charles Riley <cri...@dpspa.com>; histonet@lists.utsouthwestern.edu
>Subject: Re: [H
: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue processing question
Hello all,
I was wondering what everyone uses to secure biopsy and scant tissues through
processing. Also what would you recommend placing breast cores in for
processing. Having an argument with grossing staff and pathologist abou
Sponges can cause a compression artifact leaving some sort of "imprint" on the
surface of the biopsy, especially kidney and prostate Bx.I my experience tissue
paper is the best option. If you are having difficulties with the wrapping, you
can use "tea bags".René
On Friday, January 29,
We are moving out Peloris tissue processors to another room, so we have to
validate. I was wondering what other labs do as a validation for this type of
move? Do you run tissue on every progam utilizing each processor for at least
one of the protocols? Any help would be appreciated.
thanks,
This seems like overkill. Just moving a processor previously validated should
not require re-validation, just verify the programming and make certain the
paraffins are melted completely.
This may not apply to all processors, but for all Sakura machines manufactured
since 1980,I would not
, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-Original Message-
From: Ann Specian via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, 22 July 2015 1:06 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue processing validation
We are moving
Hello!
May I ask for your recommendations for tissue processing methods for
laser microdissection and subsequent RNA isolation?
I can think of at least the following protocols, each with significant
drawbacks (and questions):
1) Traditional FFPE sections:
+ easy handling
+ RNA is safe (but
If you fix adequately (no NBF) you can go with 1
René J.
From: Mikael Niku mikael.n...@helsinki.fi
To: histonet@lists.utsouthwestern.edu
Sent: Wednesday, February 13, 2013 8:15 AM
Subject: [Histonet] Tissue processing for laser microdissection and RNA
isolation?
Hello!
May I ask for your
Hi All,
My lab has been doing some fun histology with oyster tissues embedded in
paraffin for an upcoming paper we plan to publish. I've got about 50 samples
left that need to be processed and embedded but sadly our histology lab on
campus has had their main automated tissue processor break
Fellow histonetters, I'm looking for evidence based or best practice/
benchmarked tissue processing protocols for the small biopsies listed below.
Please provide a reference since our facility strives for evidence based
procedures for our patients .
I am processing a number of:
-
We use Mucicarmine placed in a small dropper bottle, it does not wash
out in the processor and makes the tissues easy to see once embedded.
Lisa White, HT(ASCP)
Supervisory HT
James H. Quillen VAMC
PO Box 4000
Corner of Veterans Way and Lamont
PLMS 113
Mountain Home, TN 37684
Hi,
Please let me know the name and ordering information of the blue dye used
during processing. Currently we use eosin in all our alcohols but still tissues
are very pale and hard to see at embedding.
Please share If anyone has any other sugesstions that will help to see small
tissues
We have several tissue blocks with cells that have shrunken. The pathologist
called them shrink o cytes. The tissue affected is mostly small gastric
polyps. This sounds like a dehydration issue. Any suggestions would be
greatly appreciated.
Thank you,
Carol
Carol Bryant, CT (ASCP)
You are right, it is most likely a dehydration issue.
Try to start with 70EthOL and increase gradually until 100EthOL
René J.
--- On Thu, 7/14/11, Carol Bryant cb...@lexclin.com wrote:
From: Carol Bryant cb...@lexclin.com
Subject: [Histonet] tissue processing problem
To: histonet
Dear Histonetters,
We were asked to process Hamster tissue(Large intestine) for one of our
investigators. I would like to know whether any of our users process hamster
tissues in their labs, if so kindly provide me a protocol for the same. Our
tissue processor is Sakura VIP6.
Thanks for all
Hi everyone,
I need some advice.
I have been doing tissue processing (on an old Shandon Hypercenter),
embedding and sectioning for 15 yrs. and never really had any problems.
I recently processed mouse tissues on a brand new tissue processor
(Leica ASP300S). When I went to cut 5 micron
...@lists.utsouthwestern.edu on behalf of Sheri Kelemen
Sent: Wed 3/17/2010 4:05 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] tissue processing
Hi everyone,
I need some advice.
I have been doing tissue processing (on an old Shandon Hypercenter),
embedding and sectioning for 15 yrs
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