[Histonet] troubleshooting Oil Red O

2011-08-29 Thread Denise G Crowley
Hi all, I'm using the Churukian method posted by Gayle Callis a few years ago and I have encountered a strange problem. The lipid stains fine, and the Gill's hematoxylin looks good before I coverslip with Vectashield Hard Set aqueous mounting medium. But within a matter of minutes, the

Re: [Histonet] troubleshooting Oil Red O

2011-08-29 Thread Geoff McAuliffe
It may be that Vector has changed the formulation for Hard Set. I suggest you call them up. I have found their technical support to be excellent. Geoff On 8/29/2011 10:30 AM, Denise G Crowley wrote: Hi all, I'm using the Churukian method posted by Gayle Callis a few years ago and I have