Hi Donna,
I had a time with this one as well. I use Dako's antibody on the Leica
Bondmax. I run it at 1:50 using ER2 for 30 minutes. I would be careful going
any stronger on the dilution, I got quite a bit of background with a 1:40, the
1:50 stains clean but can be a little weak if you don't
Any protocol recommendations for MYOD1? I am having a difficult time in
trying to work up this antibody.
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