AW: [Histonet] FISH enumeration

Pathologists do FISH reading. Learning by doing. Manual enumeration. Each doctor, who orders a FISH has to read it by him/herself. Often they do it with a second person/second look for reliable results. Gudrun Lang Histolab, Linz, Austria -Ursprüngliche Nachricht- Von: histonet-boun...

AW: [Histonet] FISH baking and dewaxing

We see better results with FISH-slides baked over night. This is a phenomenon called aging, known from cytogenetics. It seems, that the time of air-drying and heat leads to a better access of the probes to the dna. Therefor we let them also in the oven over the weekend. Gudrun -Ursprüngliche

AW: [Histonet] FISH

Hi Wassan, you may depart the FISH procedure in a tissue-dependant first part and a probe-dependant second part. The first part comprises the pretreatment of the tissue to retrieve and permeabilises it. The amount of pretreatment is dependant on the fixation-duration of the tissue. The longer the f

AW: [Histonet] FISH procedures

I work in a histolab in Austria as Biomedical Scientist. There's no differentiation between histotechs and MTs in this lab. We perform manual FISH on FFPET like Her2neu and others. The reading is done by pathologists. I think FISH belongs to high level duties in histolab and should be performed by

AW: [Histonet] FISH Qualifications/Training

Here it depends on the cases and the type of probes, that have to be read. In some pathology departments in Austria Her2neu is counted by histotechnologists, formerly trained by pathologists. Hematology probes for translocations in lymphomas, or FISH in difficult tissues like sarcomas are read by p

AW: [Histonet] FISH on B.M.

Miriam, do you do FISH on bone marrow trephine biopsies, that have to be decalcified before cutting and staining? If decalcifing is performed with acid like formic acid or even hydrochloric acid, DNA is degraded to smaller fragments. Successfull hybridization of this fragments is kind of chance, b

AW: [Histonet] FISH together with IF

I've just found a publication, where the IF is done before FISH. Look at this: http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=1770804&blobtype=pdf bye Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]

AW: [Histonet] FISH together with IF

Hi Wladislav, this technique is called FICTION. I'm sure, that you have done a literature search on this. If you need pepsin for your FISH depends on the fixation-status of the tissue for permeabilization and digestion of the histon-proteins. I guess formaldehyde fixed tissue cannot do without it.