mcaul...@umdnj.edu]
Sent: Wednesday, December 09, 2009 11:16 AM
To: Nicholas David Evans
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [HISTONET] fixation and storage of tissue for TEM
Fix in buffered glutaraldehyde for 2 hours, overnight is acceptable.
After multiple rinses, store in buffer i
Fix in buffered glutaraldehyde for 2 hours, overnight is acceptable.
After multiple rinses, store in buffer in the refrigerator. Change the
buffer once a week, there are things that will grow in cacodylate
buffer. You may complete osmication if you want, then rinse and store in
buffer. I would
Overnight in glutaraldehyde is more than adequate for the tiny (<1mm) bits of
tissue that are needed for transmission electron microscopy. Two hours is often
sufficient. The fixation, washing, postosmication, propylene oxide (if used)
and preliminary infiltration with catalyzed epoxy resin can t
