25-2-2010 16:42
Aan: histonet@lists.utsouthwestern.edu; histonet.nos...@vneubert.com
Onderwerp: Re: [Histonet] [ SOLVED ][ Histonet ] Strange circles in IHC slides
To me it seems that the sections after being picked from the water bath were
not completely drained and the dewaxing process
Hello,
I'm glad that you already solved the problem your way.
I didn't read your first post, but we had exactly the same problem (and
we're also using cover plates). This artifact is very likely caused by tiny
air bubbles which are trapped under the cover plate. The crucial step is
when you drip
To me it seems that the sections after being picked from the water bath were
not completely drained and the dewaxing process was incomplete in a way that
the round areas kept certain amount of paraffin wax that prevented the
reagents reactions.
The fact that the areas are round are an
