Billie,
We use Dianova's antibody and purchase it from Fisher. We've performed this
stain for a little over six months and seem to be having success with the
following protocol:
Dianova's IDH1 R132H Antibody, Diluted 1:100
Ventana Benchmark XT, iView Detection
Standard CC1
37 degrees C
32
We run it on Leica's Bond Max with good results. We use the mouse mAb (Clone
H09) from dianova at a dilution of 1:50 (15' incubation) following low-pH
retrieval.
Richard
Richard W. Cartun, MS, PhD
Director, Histology Immunopathology
Director, Biospecimen Collection Programs
Assistant
Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roger Heyna
Sent: Friday, February 22, 2013 4:52 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Antibody IDH1
Billie,
We use Dianova's antibody and purchase
