I also use the CryoJane and have problems similar to yours. I get much better
bone sections with it, but they never seem to be perfect like others say they
get. I also have tried many different ways to do it, but there always seems to
be a part that does not completely transfer from the tape
I use the tape transfer system. What tissues are you trying to cut? If you
are cutting soft tissues you should be using the 0.5 or 1x coated slides,
the 4x and above are for bone.
Have you tried putting your slides on a block of dryice after exposing them
to uv, let them sit for a while to get
I forgot to mention that I always use a D profile tungsten carbide permanent
knife to make these sections.
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net
www.ihcrg.org
-Original Message-
From: Patsy Ruegg
Hi Douglas,
We have a cryojane system, but do not use it too often. So this might not be
expert advise, but I do remember that we would sometimes flash the slide more
than once. I was just checking in the procedure manual that came with the
equipment and it does not mention to do this.
