Yes, I am also using pH 6 HIER with 10mM sodium citrate, 0.05% Tween-20. I
do 20 minutes at 95 degree C to retrieve BrdU on mouse embryo tissues. I
use Abcam ab6326. Their product sheet states that you may used heat for
antigen retrieval.
The BrdU epitope needs to be revealed by denaturing the dou
I used pH 6 HEIR instead of HCl/enzyme for BrdU - it works very well on mouse
and monkey intestine. Did not switch to HCl after that. But now I prefer to use
EdU instead of BrdU (Click-iT kit from Invitrogen). It is more expensive, but
much more sensitive, fast, easy to use and well compatible w
You can also perform BrdU on the MMA sections the BrdU label is quite hardy.
Robert Schoonhoven
From: Nancy W. Troiano
To: histonet@lists.utsouthwestern.edu
Sent: Tuesday, April 21, 2009 1:37:01 PM
Subject: [Histonet] BRDU, Tetracycline, calcein in bone
I wou
Patsy,
If the animal was treated with BrdU initially, then yes I have. Unfortunately
All of my papers and reference materials are boxed up at the moment (preparing
to move) but I have done this procedure so many times that I can pretty much
reconstruct it from memory.
1) Hydrate slides to dd
I used proteinase Kfor Tunel antigen retrieval...
2009/4/15 TF
> Hi all, in many IHC co-labeling with BrdU, we perform the other antigen
> first.
> How about TUNEL?
> Do BrdU antigen retrieval (HCL or citrate buffer) affect TUNEL staining?
> Thanks very much.
>
> 2009-04-15
>
>
>
> TF
>
Abcam monoclonal rat-anti BrdU
http://www.abcam.com/BrdU-antibody-BU1-75-ICR1-ab6326.html
Just compare to other Abcam anti-BrdU, it has most citations at least.
2009-03-18
TF
发件人： Amy Lee
发送时间： 2009-03-18 07:14:56
收件人： tifei
抄送：
主题： Re: [Histonet] BrdU immunostain on rat brain
hi, we worked a lot on this.
I prefer to use 40 um frozen sections.
Abcam anti-BrdU (rat sourced) is the best ever I know. It does not have high
background with rat IgG. Some other mouse-/sheep- sourced BrdU are available.
2009-03-17
TF
发件人： Amy Lee
发送时间： 2009-03-17 05:02:28
收件人： hist
hi,
1. no idea.
2. yes. if they proliferate.
3. just treat with PBST (Tris-buffered PBS) before your adding of antibody.
4. possibly..just notice some BrdU positive cells could be microglia..be
careful of the leakage..and if you dont have a GFP-rat, have you considered of
using astrocytes from mi
