Re: [Histonet] Blades for cutting resin on a microtome

2014-09-12 Thread Jack Ratliff
Veronique, May I ask what type of specimen is embedded into the JB-4 resin? Nevertheless, you should be able to cut these blocks using a tungsten-carbide knife. While there are a few vendors out there that sell these knives, in my laboratory I personally use knives re-sharpened by Delaware

RE: [Histonet] Blades for cutting resin on a microtome

2014-09-12 Thread Keyser Gerald T
I've only cut resin with a glass or diamond knife in an ultramicrotome. If you are attempting to do it in a regular microtome, you would need a special blade holder. I don't know if any microtome manufactures make glass knife holders. You make the glass blades yourself using special glass.

Re: [Histonet] Blades for cutting resin on a microtome

2014-09-12 Thread Alan Bright
Yes brightinstruments, com make glass knife holder and tungsten carbide tipped knives for microtomes, KR,Alan Bright Sent from my iPhone On 12 Sep 2014, at 15:49, Keyser Gerald T gkey...@uwhealth.org wrote: I've only cut resin with a glass or diamond knife in an ultramicrotome. If

RE: [Histonet] Blades for cutting resin on a microtome

2014-09-12 Thread Patsy Ruegg
with them. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com pru...@ihctech.net From: abri...@brightinstruments.com Date: Fri, 12 Sep 2014 17:32:47 +0100 To: gkey...@uwhealth.org Subject: Re: [Histonet] Blades