Orla,
You seem to have introduced many poor histologic processing practices into your
technique.
Firstly ???4% paraformaldehyde. How did you make this up? The nomenclature is
incorrect (see Histonet archives and Manoonkitiwongsa PS, Schultz RL. (2002)
Proper nomenclature of formaldehyde and
Orla
I know this might sound silly but normal mouse bone marrow contains both fat
and hemoatopoietic marrow, the areas that are fat will appear like round
voids. Is that what you are seeing?
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
