We borrowed a wonderful cell block procedure from Windham Community Memorial
Hospital here in CT several years ago. The specimen is collected fresh in
sterile saline or RPMI and then centrifuged to concentrate the cells. Plasma
and then thrombin are added to form a clot. The clot is then
This is how we do it now. In the old days, we used agar and to my mind, it is
still the best way when you have scant material.
- Spin in a conical tube and pour off
- Melt an agar slant (we get TSA slant from micro)
- Pour the agar into the conical tube and spin for 5 minutes
- The agar will
'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Cell Block Preparation
This is how we do it now. In the old days, we used agar and to my mind, it is
still the best way when you have scant material.
- Spin in a conical tube and pour off
- Melt an agar slant (we get TSA slant from micro
Of
Rathborne, Toni
Sent: Friday, September 06, 2013 7:18 AM
To: 'Tom McNemar'; 'Ann Specian'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Cell Block Preparation
I wonder if this method could be used with the product Histogel. Has
anyone tried it?
-Original Message-
From: histonet-boun
We have had mixed results in the past with cell blocks, but currently we
are filtering the fluid through a biopsy bag and processing that. We
seem to be retaining more specimen with that method (we have used
sponges and lens paper in the past).
Michael J. Dessoye, M.S. | Histology Supervisor |
Medical Center
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dessoye,
Michael J
Sent: Friday, September 06, 2013 9:39 AM
To: Ann Specian; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Cell
We spin the sample down, pour off supernatant. Resuspend in 5 drops
thromboplastin and 4 drops human serum we receive from our phlebotomy lab.
Let firm for 5 minutes and then fix in NBF. The agar suggestion probably
works similarly if you can't get serum.
On Sep 6, 2013 9:39 AM, Dessoye, Michael J
