Re: [Histonet] Embedding

2024-01-03 Thread Jay Lundgren via Histonet
My lab uses trained lemurs, so I certainly hope not. Virus-free.www.avast.com

Re: [Histonet] Embedding station

2017-03-22 Thread Rene J Buesa via Histonet
"Open" your options and try Sakura.René On Tuesday, March 21, 2017 3:54 PM, "Flynn, Evelyn via Histonet" wrote: Hello all,   Our laboratory is purchasing a new paraffin embedding station.  We are considering a Leica Arcadia or a Thermo HistoStar

Re: [Histonet] embedding and microtomy "medical waste"

2016-05-11 Thread Morken, Timothy via Histonet
Curt, we went through the same thing, but for the embedding we keep a plastic bucket on the bench and lined with a biohazard bag, and put all the lids and papers in that. We keep those for a week in case some tissue ends up missing. Then it goes into the red cans. At the microtome we do use

Re: [Histonet] embedding center backup

2015-05-18 Thread Rathborne, Toni
Stacy, Our volume is similar, and we do have a backup embedding center. I'm sure that you could get a used one for much less than a new one to have just for this purpose. Toni -Original Message- From: Stacy McLaughlin [mailto:stacy_mclaugh...@cooley-dickinson.org] Sent: Monday, May

Re: [Histonet] Embedding Question

2015-03-12 Thread Pam Marcum
I am sorry anytime you are carrying patient tissue in cassettes (processed or not) is a bad plan.  In order to guarantee the tissue is safe it would need to be enclosed in a way that an accidental bump or fall could not allow tissue cassettes to go flying off down the hall.  Playing 52 card

Re: [Histonet] Embedding Question

2015-03-12 Thread Rene J Buesa
Whom ever made the decision (probably an administrator) is totally ignorant of what histology flow is all about.To put it mildly it is an absolutely stupid decision.Has this decider any idea about Lean, sure not.To have a good work flow everything should be as close as possible.René J. 

RE: [Histonet] Embedding Question

2015-03-12 Thread Podawiltz, Thomas
Not what I call a lean system. Why the change? Tom Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare 603-524-3211 ext: 3220 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello

RE: [Histonet] Embedding Question

2015-03-12 Thread Morken, Timothy
Paula, How many times per day? Is the embedding close to the cutting area? Of course any extra walking is a problem, especially in busy areas. Is this a non-patient area (hopefully!)? Any restructuring should be to move things closer together, not further away! Having said that, If it comes

RE: [Histonet] Embedding Question

2015-03-12 Thread O'Donnell, Bill
Wow. Speechless. (well nearly speechless) Wow. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Thursday, March 12, 2015 8:29 AM To: HistoNet Subject: [Histonet] Embedding Question It

Re: [Histonet] Embedding Question

2015-03-12 Thread Paula Sicurello
] On Behalf Of Podawiltz, Thomas Sent: Thursday, March 12, 2015 10:32 AM To: Paula Sicurello; HistoNet Subject: RE: [Histonet] Embedding Question Not what I call a lean system. Why the change? Tom Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare 603-524-3211 ext: 3220 -Original

Re: [Histonet] Embedding Question

2015-03-12 Thread Lucie Guernsey
If I may, I'd like to piggy-back onto what Paula has mentioned regarding allowing paraffin infiltrated tissue to cool before embedding it. Hopefully someone can help both of us out, even if we seem to warm our infiltrated tissue differently (Paula's in a dry bin and mine in a wax bath). I work in

RE: [Histonet] Embedding Question

2015-03-12 Thread Podawiltz, Thomas
: Thursday, March 12, 2015 1:22 PM To: Lucie Guernsey Cc: HistoNet; Morken, Timothy Subject: Re: [Histonet] Embedding Question Cooling the paraffin and then re melting will not affect the tissue, unless the combined heated, liquified state period becomes extended. Cooling the paraffin is a great

Re: [Histonet] Embedding Question

2015-03-12 Thread WILLIAM DESALVO
Cooling the paraffin and then re melting will not affect the tissue, unless the combined heated, liquified state period becomes extended. Cooling the paraffin is a great protector of the tissue, no different than what you have with a completed block. Be cautious at how fast and at what

Re: [Histonet] Embedding Question

2015-03-12 Thread Sue
I guess my first question would be why?  As far as the paraffin frezzing, we leave out biopsies out and when ready to embed we place them on the hot area of the embedding center until they are melted then embed and have no issues at all.   The only thing I can think of is the area they are

RE: [Histonet] Embedding Question

2015-03-12 Thread Jeffrey Robinson
, Clovis, CA. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Thursday, March 12, 2015 9:47 AM To: Hannen, Valerie Cc: HistoNet Subject: Re: [Histonet] Embedding Question Why the change

RE: [Histonet] Embedding Question

2015-03-12 Thread Marcum, Pamela A
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey Sent: Thursday, March 12, 2015 12:08 PM To: Paula Sicurello Cc: HistoNet; Morken, Timothy Subject: Re: [Histonet] Embedding Question If I may, I'd like to piggy-back onto what Paula has mentioned regarding allowing

Re: [Histonet] Embedding Question

2015-03-12 Thread Paula Sicurello
To: Hannen, Valerie Cc: HistoNet Subject: Re: [Histonet] Embedding Question Why the change? Space. There is a larger space available for histology. It would be optimum to either move the processors into the new space or leave the embedding centers where they are now. I mentioned did bring

Re: [Histonet] embedding of insects in historesin (Technovit 7100)

2014-11-23 Thread Damien
Hi Jurgen, If you still need assistance,please let me know. Feel free to contact me directly. Best, Damien L. On Fri, Nov 21, 2014 at 12:55 PM, dr.ha...@gmx.net wrote: Hallo, Andi G. Thanks for your reply. Normally I use paraffine for embedding insects after dehydration and

RE: [Histonet] embedding of insects in historesin (Technovit 7100)

2014-11-21 Thread Grantham, Andrea L - (algranth)
First of all, this sounds like a Damien Laudier question. Damien, I hope you can help this guy. Secondly, do you have to use plastic embedding? I was successful with paraffin embedding. I was able to section various small insects and also bees. It takes some patience and soaking in mollifex or

RE: [Histonet] embedding of insects in historesin (Technovit 7100)

2014-11-21 Thread Dr . Harst
Hallo, Andi G. Thanks for your reply. Normally I use paraffine for embedding insects after dehydration and using N Butylalkohol with sucess. In my opinion it is absolutly necessary to avoid any rests of water and to cut cool. But this technique is limited to sections of 3-4

Re: [Histonet] Embedding paraffin for brain tissue?

2014-01-02 Thread Pirici Daniel
Street 2, 200349 Craiova, Dolj Romania From: Maria Mejia mbmph...@gmail.com To: Tim Wheelock twheel...@mclean.harvard.edu Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Thursday, January 2, 2014 8:04 AM Subject: Re: [Histonet

Re: [Histonet] Embedding paraffin for brain tissue?

2014-01-01 Thread Maria Mejia
Hello, I too have been experiencing the same difficulties cutting brain samples, I've tried 3 different types of paraffins - all the the same rolling of sections, some lines tearing. It's not my microtome, because I've had it checked it went through a recent general maintenance. I've tried

RE: [Histonet] Embedding cells in Paraffin

2013-12-02 Thread joelle weaver
I spun down the cultured cells and then used a product called histogel. It kept them protected through the VIP, paraffin embedded like cell block buttons and was able to cut regular paraffin sections. I believe that I purchased the histogel from American Mastertech. Joelle Weaver MAOM, HTL

Re: [Histonet] Embedding Paraffin

2013-11-13 Thread Kim Donadio
We use formulae R from Leica for both infiltrating and embedding with excellent results. Sent from my iPhone On Nov 12, 2013, at 10:02 AM, Matthew D. Roark mro...@sfmc.net wrote: What paraffin does everyone like for embedding? We are currently using Surgipaths EM-400 but its dirty!

Re: [Histonet] Embedding Paraffin

2013-11-12 Thread Pam Marcum
We use Polyscientific RD Paraffin Prills and love it.  The price has been excellent and it is a very clean product.  Too ma ny changes in other companies leading to base product issues I just did not have the time or patience for, so I went with the best I could find.  I used it at the

Re: [Histonet] Embedding problem

2013-08-02 Thread Benjamin
Plastic or metal molds? Sent from my iPhone On Aug 1, 2013, at 11:26 AM, Tim Wheelock twheel...@mclean.harvard.edu wrote: Hi Everyone: When I paraffin embed brain tissue, I gentle push down on all regions (especially the edges) of the specimen(s). It seems that all of the areas of the

RE: [Histonet] Embedding SOP

2013-08-02 Thread joelle weaver
How Sheehan Hrapchak, and any gross dissection manual for inking/anatomical orientation guidelines? I put in diagrams and tissue and biopsy site specific instructions, and I think this helps immensely in keeping blocks consistent and creating standardization. Joelle Weaver MAOM, HTL

Re: [Histonet] Embedding Contamination

2013-07-24 Thread Rene J Buesa
This how I always handled this issue: 1- document the contamination in your QC file trying to identify the tissue source; 2- train the embedding histotechs in the proper way of cleaning the embedding instruments and the forceps wells and document it also in the corresponding QC file; 3- save

Re: [Histonet] Embedding Contamination

2013-07-24 Thread Jay Lundgren
Everyone jumps on the embedder in these situations, but the contamination might be coming from the gross board or the tissue processor as well. So be sure to train the appropriate docs/PA's/histotechs how to avoid cross contamination when grossing/processing as well. And stop jumping on

RE: [Histonet] Embedding Centers

2012-12-06 Thread Burton, Lynn
Both animal disease labs in the state of Illinois have used the Sakura machines for the twenty years I have been here with great success. We also have aSakura processor that has been going for 25 years and a coverslipper that has only had 3 service calls for minor problems in the past 15+

Re: [Histonet] Embedding beads

2012-07-02 Thread Jennifer Campbell
The identifier(whatever is using to distinguish who did the task) is not embedded with the specimen. It is put in the top portion of the cassette. At least that is what we have always done here. On Mon, Jul 2, 2012 at 1:17 PM, Amber McKenzie amber.mcken...@gastrodocs.net wrote: How do you

Re: [Histonet] Embedding beads

2012-07-02 Thread Alan Taylor
Hi All The embedding bead goes into the cassette tray, into one corner, not into the stainless steel embedding mould with the tissue. The plastic cassette is placed on top of the mould, as usual and filled with molten wax, the coloured bead is quickly dropped in and the embedding mould is

Re: [Histonet] Embedding beads

2012-06-25 Thread Jennifer Campbell
@lists.utsouthwestern.edu Subject: Re: [Histonet] Embedding beads Hi All We use Hamma beads to identify individual embedders on a particular day. Hamma beads will be well known to parents and child carers as tiny tube like beads that can be made into many patterns and then ironed (by adults) to make

Re: [Histonet] Embedding beads

2012-06-25 Thread Grantham, Andrea L - (algranth)
I called on a lab in Las Vegas when I had my sales job that used sequins. Well, it was Vegas! Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724

RE: [Histonet] Embedding beads

2012-06-25 Thread Victor A. Tobias
: [Histonet] Embedding beads I called on a lab in Las Vegas when I had my sales job that used sequins. Well, it was Vegas! Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724

Re: [Histonet] Embedding beads

2012-06-25 Thread Alan Taylor
Message - From: Jennifer Campbell To: Amber McKenzie Cc: Alan Taylor ; Willis, Donna G. ; histonet@lists.utsouthwestern.edu Sent: Monday, June 25, 2012 2:51 PM Subject: Re: [Histonet] Embedding beads We use squares of colored construction paper. Each tech is assigned a color

Re: [Histonet] Embedding beads

2012-06-22 Thread Alan Taylor
Hi All We use Hamma beads to identify individual embedders on a particular day. Hamma beads will be well known to parents and child carers as tiny tube like beads that can be made into many patterns and then ironed (by adults) to make colourful place mats etc for children. Hamma beads come

RE: [Histonet] Embedding

2012-05-30 Thread Stephenson, Sheryl
That was my initial thought, check your paraffin. I could be coming from the paraffin.. or whatever you are cleaning with make sure its dust free/debris free. Sheryl Stephenson | Histology Technician Main 908.947.1100 Fax908.947.1085 Direct:

RE: [Histonet] Embedding

2012-05-25 Thread Norton, Sally
We make sure to clean the wells also. Little flecks of tissue are almost always in there after embedding. Sally Norton Seattle Children's -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Specian

Re: [Histonet] Embedding

2012-05-25 Thread Ann Specian
1:12 pm Subject: RE: [Histonet] Embedding We make sure to clean the wells also. Little flecks of tissue are almost always n there after embedding. Sally Norton eattle Children's -Original Message- rom: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun

Re: [Histonet] embedding

2012-05-24 Thread Rene J Buesa
60 René J. --- On Thu, 5/24/12, cindy dewar cindy38...@yahoo.com wrote: From: cindy dewar cindy38...@yahoo.com Subject: [Histonet] embedding To: histonet@lists.utsouthwestern.edu Date: Thursday, May 24, 2012, 3:01 PM On average, how many blocks should a tech with 6 years experience be able to

RE: [Histonet] embedding

2012-05-24 Thread McMahon, Loralee A
[rjbu...@yahoo.com] Sent: Thursday, May 24, 2012 3:55 PM To: histonet@lists.utsouthwestern.edu; cindy dewar Subject: Re: [Histonet] embedding 60 René J. --- On Thu, 5/24/12, cindy dewar cindy38...@yahoo.com wrote: From: cindy dewar cindy38...@yahoo.com Subject: [Histonet] embedding To: histonet

RE: [Histonet] embedding centers

2011-09-13 Thread Sherwood, Margaret
Ditto. Sakura's Tissue-Tek Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org -Original

Re: [Histonet] embedding centers

2011-09-13 Thread Sean McBride
Double ditto. Sakura TEC ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412-268-8275 (fax) smcbr...@andrew.cmu.edu On Sep 13, 2011, at

Re: [Histonet] embedding centers

2011-09-13 Thread Rene J Buesa
As you correctly point out, all are of similar configuration and foot-print. All use the same technology (a cooling area, a heating bath and a hot compartment). As to reliability TissueTek if the best for me. There are some more than 25 years old still providing a good and reliable service.

RE: [Histonet] embedding centers

2011-09-13 Thread Bartlett, Jeanine (CDC/OID/NCEZID)
...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, September 13, 2011 10:05 AM To: histonet@lists.utsouthwestern.edu; StephanieWeaver Subject: Re: [Histonet] embedding centers As you correctly point out, all are of similar configuration and foot-print. All use the same technology

Re: [Histonet] embedding centers

2011-09-13 Thread Nicole Tatum
Stephanie, I prefur the Sakura Tissue-Teks. III being my fav, but it is becomming out dated and hard to find parts for. V is good to. The Leica brand is always a winner. Lots of people have these, so there easy to get parts and have maintenced. Make sure you check and see how many cassettes the

Re: [Histonet] embedding centers

2011-09-13 Thread Jill Cox
I agree! We just sent our brand new Medite back to vendor because of problems. The cold spot was icing over and dispenser was dispensing paraffin when I wasn't even using it! We just purchased the Sakura tec which has been my favorite since I've been a tech. Sent from my iPhone On Sep 13,

RE: [Histonet] Embedding Center Questions

2011-08-26 Thread Rathborne, Toni
A couple of places that come to mind are Belair (800-783-9424), Medical Equipment Source (www.medequipsource.com), and Mayflower (800-287-9801). I know that they sell used equipment, you could inquire about a rental. Good luck. -Original Message- From:

RE: [Histonet] Embedding process improvement and competency assessment

2011-08-25 Thread Heath, Nancy L.
sdatt...@stormontvail.org Subject: RE: [Histonet] Embedding process improvement and competency assessment To: Rene J Buesa rjbu...@yahoo.com, histonet@lists.utsouthwestern.edu Date: Wednesday, August 24, 2011, 5:14 PM Rene', I agree with many of the points you make about a non-histotech managing

RE: [Histonet] embedding

2011-08-25 Thread joelle weaver
I also like this reference- I like the quote. Quality embedding is key to me to the ultimate production for high quality, and sometimes discounted and overlooked in that regard. Joelle Weaver MAOM, BA, (HTL) ASCP Date: Thu, 25 Aug 2011 09:29:17 -0700 From: kdboydhi...@yahoo.com To:

RE: [Histonet] embedding

2011-08-25 Thread Garrison, Becky
I would love to have a copy of your embedding guidelines. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] Embedding process improvement and competency assessment

2011-08-24 Thread joelle weaver
Some time ago I did a NSH teleconference on process improvement, and chose to apply the methodology to the embedding task. I had a handout for measurement of embedding competency and also training information( skills needed and assessment ideas), with specific discussin on tissue orientation

Re: [Histonet] Embedding process improvement and competency assessment

2011-08-24 Thread joelle weaver
I do concur that assessing any task is certainly much more straightforward if you are starting from your personal knowledge base and experience, but maybe with the input of others?, could still be accomplished ( thinking positive, through a team approach)- Sent from my Verizon Wireless

RE: [Histonet] Embedding process improvement and competency assessment

2011-08-24 Thread D'Attilio, Shelley
Rene', I agree with many of the points you make about a non-histotech managing a histology laboratory, especially since the decision could be seen to devalue the special training and knowledge of a histotech. Every bit of knowledge I have gained along the way has been hard-fought, to say the

RE: [Histonet] Embedding process improvement and competency assessment

2011-08-24 Thread Bea DeBrosse-Serra
I have to agree with René. I have worked under registered histologists and MT's. And under certain circumstances it is EXTREMELY hard to make a non-histologist to understand what our needs are. But at least it appears to me, that you want to learn and are open to understand the histology way.

Re: [Histonet] Embedding Media

2011-07-08 Thread histot...@imagesbyhopper.com
We use the infiltration embedding paraffin from Surgipath/Leica and are very happy with it. Michelle Sent from my iPhone On Jul 8, 2011, at 12:44 PM, Paula Lucas plu...@biopath.org wrote: Hello, We are considering a switch to a different brand of paraffin and this is because I feel

RE: [Histonet] Embedding multiple GI pieces on end in a paraffin block

2010-07-16 Thread sgoebel
How many are we talking about? I embed 6 sections of mouse = bowel on end and it works? Just fill the mold, put it on the cold spo= t for a second, then on some room temperature area, the paraffin will harde= n slowly enough that you should be able to embed them? Sarah Goebel,

RE: [Histonet] Embedding multiple GI pieces on end in a paraffin block

2010-07-16 Thread Mike Pence
I have techs that embed 10-15 pieces on end in one block. Just cool the block slowly and move your pieces around quickly. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kgrob...@rci.rutgers.edu Sent:

Re: [Histonet] Embedding multiple GI pieces on end in a paraffin block

2010-07-16 Thread Drew Meyer
We regularly embed 6-12 pieces on end in one block without any special method. You just have to be quick... and don't leave the block on the cold plate very long... just touch the cold plate briefly while embedding the individual piece, then lift the block off the plate until you grab the next

RE: [Histonet] Embedding multiple GI pieces on end in a paraffin block

2010-07-16 Thread kgrobert
To all, OK, looks like most of you are saying the same thing-work fast! :o) I've printed out all of your replies and discussed them with the graduate student who needs this done for her thesis, and she agrees with me-it's just going to take practice. I'll try the cool slowly/work fast

RE: [Histonet] Embedding multiple GI pieces on end in a paraffin block

2010-07-16 Thread R J VAZQUEZ
Date: Fri, 16 Jul 2010 11:00:01 -0500 From: mpe...@grhs.net To: kgrob...@rci.rutgers.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Embedding multiple GI pieces on end in a paraffin block CC: I have techs that embed 10-15 pieces on end in one block. Just cool the block

RE: [Histonet] Embedding Beads

2010-04-03 Thread WILLIAM DESALVO
I suggest trying a less expensive and easy to use identifier, party confetti. You can purchase on the internet and buy individual packages in specific shapes and colors. We have over 50 individuals w/ an assined shape/color and it works great. www.shindigz.com www.chicoparty.com

Re: [Histonet] embedding method for DRGs

2010-03-28 Thread John Kiernan
Dear Carol Barone, You are asking the wrong group (Histonet), and almost anonymously. Your email address indicates that you work for a BIG company. Your employer should send you on a course to learn how to do microdissection of fetal and neonatal mice. You may get some free advice from

RE: [Histonet] Embedding Forceps

2010-03-08 Thread Harlem Kaputnik
Use the appropriate tool for the job needed... you don't want to damage the tissue. For the pointy/sharp forceps, just use more of a delicate touch (this comes with practice)... they have the blunt tipped forceps that work great for skin. Date: Mon, 8 Mar 2010 11:27:41 -0800 From:

RE: [HISTONET] embedding cell cultures

2010-01-28 Thread Jo Dee Fish
Hi Nick, If you can, grow the cells on thermanox cover slips (NUNC). Then process them as normal for embedding in Eponate 12. I use acetonitrile in place of the propylene oxide to avoid damaging the coverslip. When doing the final embedding and polymerization, invert the coverslip over a drop

Re: [HISTONET] embedding cell cultures

2010-01-27 Thread Geoff McAuliffe
Hi Nick: You can use the Epon substitutes such as EmBed 812. Fix, osmicate, dehydrate as usual, but omit the proplylene oxide as it will react with the plastic dish. Epon substitutes will mix with ethanol. I used 2:1 then 1:1 then 1:2 ratios of absolute ethanol to Epon with catalyst added

RE: [HISTONET] embedding cell cultures

2010-01-27 Thread Sherwood, Margaret
...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe Sent: Wednesday, January 27, 2010 3:20 PM To: Nicholas David Evans Cc: histonet@lists.utsouthwestern.edu Subject: Re: [HISTONET] embedding cell cultures Hi Nick: You can use the Epon substitutes

Re: [HISTONET] embedding cell cultures

2010-01-27 Thread Peggy Bisher
, 2010 3:20 PM To: Nicholas David Evans Cc: histonet@lists.utsouthwestern.edu Subject: Re: [HISTONET] embedding cell cultures Hi Nick: You can use the Epon substitutes such as EmBed 812. Fix, osmicate, dehydrate as usual, but omit the proplylene oxide as it will react with the plastic dish

RE: [Histonet] Embedding Stamp???

2009-04-14 Thread Bartlett, Jeanine (CDC/CCID/NCZVED)
It's called a tamper and I know Fisher sells them.search embedding tamper on the web site. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] Embedding Stamp???

2009-04-14 Thread Weems, Joyce
They are called tampers and are available at Sakura - 1551 and 1552. They are also available through Cardinal, but I'm not sure the item numbers for Cardinal. Joyce -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On

Re: [Histonet] Embedding Stamp???

2009-04-14 Thread Rene J Buesa
Some people call it a thumper (I do not like the name at all) and others call it a tissue pressing tool or a tissue flattening tool. Always made of aluminum with a T shape. René J. --- On Tue, 4/14/09, Yeonju Shim shimj...@gmail.com wrote: From: Yeonju Shim shimj...@gmail.com Subject:

Re: [Histonet] Embedding Stamp???

2009-04-14 Thread Pamela Marcum
Eastern Subject: Re: [Histonet] Embedding Stamp??? Some people call it a thumper (I do not like the name at all) and others call it a tissue pressing tool or a tissue flattening tool. Always made of aluminum with a T shape. René J. --- On Tue, 4/14/09, Yeonju Shim shimj...@gmail.com wrote

RE: [Histonet] Embedding Stamp???

2009-04-14 Thread Martin, Gary
Go to a Pipe shop and look into tobacco tampers. Very useful ... they have a longer handle and don't seem quite as hot as the square Histo tamper. Gary -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yeonju

RE: [Histonet] Embedding Stamp???

2009-04-14 Thread Bill B.
Ack! You beat me to it ;-) We use pipe nails which come from a local pipe store and are very inexpensive. They work well for prostate and other core biopsies. Bill B. At 7:12 AM -0700 4/14/09, Martin, Gary wrote: Go to a Pipe shop and look into tobacco tampers. Very useful ... they have a

RE: [Histonet] Embedding Stamp???

2009-04-14 Thread McCormick, James
Histonet friends, Try using the hex head of a 3/8 inch diameter 1-2 1/2 long bolt. This works quite well and costs about 15 cents at the hardware store. J.B.McCormick, M.D. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] Embedding Stamp???

2009-04-14 Thread Andrea Hooper
Sakura used to sell items called Tissue Tampers just for this purpose in different sizes. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yeonju Shim Sent: Tuesday, April 14, 2009 8:54 AM To:

Re: [Histonet] Embedding cell pellets

2009-04-02 Thread anh2006
Histogel works well for this purpose too. --Original Message-- From: Thomas Pier Sender: histonet-boun...@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu To: louise_hart...@urmc.rochester.edu Sent: Apr 2, 2009 12:17 PM Subject: Re: [Histonet] Embedding cell pellets Fix

RE: [Histonet] Embedding cell pellets

2009-04-02 Thread Bernice Frederick
...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Pier Sent: Thursday, April 02, 2009 11:18 AM To: histonet@lists.utsouthwestern.edu; louise_hart...@urmc.rochester.edu Subject: Re: [Histonet] Embedding cell pellets Fix the cells, spin them down

Re: [Histonet] Embedding paraffin blocks

2008-12-04 Thread Rene J Buesa
Under separate cover I am forwarding data on the subject and, yes, there are differences between types of specimens and that is defined as the range of the averages in my article. René J. --- On Thu, 12/4/08, Jean Warren [EMAIL PROTECTED] wrote: From: Jean Warren [EMAIL PROTECTED] Subject:

Re: [Histonet] embedding

2008-11-06 Thread Jackie M O'Connor
2-3 hours. KELLY BOYD [EMAIL PROTECTED] Sent by: [EMAIL PROTECTED] 11/06/2008 12:25 PM Please respond to [EMAIL PROTECTED] To histonet Histonet@lists.utsouthwestern.edu cc Subject [Histonet] embedding Just trying to get some feedback on how much time (estimated) it should take an

Re: [Histonet] embedding

2008-11-06 Thread stephanie . d . rivera
Hi Kelly, I would say at least 2.5 - 3 hrs. Estimating embedding approx. 50 blocks/hr. That is reasonable. That doesn't include scraping and organizing the blocks. Stephanie D. Rivera, B.S., HT(ASCP) Scientist Safety Assessment Department GlaxoSmithKline 709 Swedeland RD King of Prussia, PA

RE: [Histonet] embedding

2008-11-06 Thread Podawiltz, Thomas
I would say 2 to 4 hours, depending on how they are submitted. Were biopsy bags or foam pads used?, size of pcs. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 From: [EMAIL PROTECTED] [EMAIL