RE: [Histonet] Embeeding frozen tissue in paraffin

2009-05-16 Thread Patsy Ruegg
] Embeeding frozen tissue in paraffin I assume you mean the tissue was frozen without fixation, not that you want to put it into paraffin without fixation? No tissue will withstand paraffin processing without fixation. The best way to turn a frozen tissue into a paraffin embedded tissue is to drop the

RE: [Histonet] Embeeding frozen tissue in paraffin

2009-05-15 Thread Monfils, Paul
I assume you mean the tissue was frozen without fixation, not that you want to put it into paraffin without fixation? No tissue will withstand paraffin processing without fixation. The best way to turn a frozen tissue into a paraffin embedded tissue is to drop the frozen tissue (either plain or

Re: [Histonet] Embeeding frozen tissue in paraffin

2009-05-15 Thread APRIL SHOWERS
> is there any good method for embeeding fresh frozen tissue (without any fixation) in paraffin? Wouldn't the molten paraffin would melt the frozen tissue? LOL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.ed

Re: [Histonet] Embeeding frozen tissue in paraffin

2009-05-15 Thread louise renton
why would you want to do this? - the water contained in the fresh tissue will not "mix" with the paraffin wax. There would be little purpose in trying. On 5/15/09, Tomasz Bonda wrote: > > Hello, > is there any good method for embeeding fresh frozen tissue (without any > fixation) in paraffin? > I